SNP discovery and molecular evolution in Anopheles gambiae, with special emphasis on innate immune system.
SNP discovery and molecular evolution in Anopheles gambiae, with special emphasis on innate immune system.
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DOI：
10.1186/1471-2164-9-227
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发表时间：
2008-05-19
期刊：
影响因子：
4.4
通讯作者：
Kafatos FC
中科院分区：
文献类型：
--
作者：
Cohuet A;Krishnakumar S;Simard F;Morlais I;Koutsos A;Fontenille D;Mindrinos M;Kafatos FC
按蚊的先天免疫影响疟原虫的发育，是创新疟疾控制战略的潜在目标。免疫基因中核苷酸多样性的程度和分布可能为了解病原体-载体相互作用的进化力量提供依据。多态性的发现是进行感染易感性关联研究的重要一步。我们对冈比亚按蚊自然种群中72个免疫相关基因的编码片段和37个随机选择的基因进行了测序，以提供整个基因组遗传多样性的背景测量。平均核苷酸多样性（π）为0.0092。gambiae S型、M型和A型的平均值分别为0.0076和0.0064。阿拉伯的。在每个物种中，免疫相关和非免疫相关基因之间的平均核苷酸多样性没有统计学显著差异。在这两类基因中检测到强大的纯化选择，大概反映了强大的功能限制。我们的研究结果表明，在A.冈比亚。我们确定的3，214个单核苷酸多态性（SNP）是第一个来自新鲜现场收集材料的大型按蚊SNP集，是未来表型关联研究的相关标记。
Anopheles innate immunity affects Plasmodium development and is a potential target of innovative malaria control strategies. The extent and distribution of nucleotide diversity in immunity genes might provide insights into the evolutionary forces that condition pathogen-vector interactions. The discovery of polymorphisms is an essential step towards association studies of susceptibility to infection. We sequenced coding fragments of 72 immune related genes in natural populations of Anopheles gambiae and of 37 randomly chosen genes to provide a background measure of genetic diversity across the genome. Mean nucleotide diversity (π) was 0.0092 in the A. gambiae S form, 0.0076 in the M form and 0.0064 in A. arabiensis. Within each species, no statistically significant differences in mean nucleotide diversity were detected between immune related and non immune related genes. Strong purifying selection was detected in genes of both categories, presumably reflecting strong functional constraints. Our results suggest similar patterns and rates of molecular evolution in immune and non-immune genes in A. gambiae. The 3,214 Single Nucleotide Polymorphisms (SNPs) that we identified are the first large set of Anopheles SNPs from fresh, field-collected material and are relevant markers for future phenotype-association studies.
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