X-to-autosome expression and msl-2 transcript abundance correlate among Drosophila melanogaster somatic tissues.
X-to-autosome expression and msl-2 transcript abundance correlate among Drosophila melanogaster somatic tissues.
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作者：
Vensko Ii SP;Stone EA
在果蝇中，雄性特异性致死（MSL）复合物已被广泛研究，其在上调雄性X-连锁基因中的作用。高通量技术的最新进展提高了我们对MSL复合物如何通过全染色体染色质修饰介导剂量补偿的理解。然而，大多数研究都集中在不能反映体内剂量补偿的任何潜在异质性的细胞系模型上。细胞系和生物体基因水平剂量补偿上调之间的比较表明体细胞组织中MSL复合物活性存在差异的可能性。我们假设的程度，但不超过2倍，其中MSL复杂上调男性X连锁基因的组织类型不同的数量。在该模型中，MSL复合物丰度充当变阻器以控制上调的程度。使用公开的表达数据，我们提供的证据，我们的模型在果蝇体细胞组织。具体而言，我们发现X-常染色体表达与msl-2的组织特异性表达相关，msl-2编码MSL复合物的必需男性特异性组分。该结果表明MSL复合物介导的剂量补偿因组织类型而定量变化。此外，这一结果的模型解释的生物规模的分子和进化的后果MSL介导的剂量补偿的后果。
In Drosophila melanogaster, the male-specific lethal (MSL) complex has been studied extensively for its role in upregulating male X-linked genes. Recent advances in high-throughput technologies have improved our understanding of how the MSL complex mediates dosage compensation through chromosome-wide chromatin modifications. Most studies, however, have focused on cell line models that cannot reflect any potential heterogeneity of in vivo dosage compensation. Comparisons between cell line and organismal gene-level dosage compensation upregulation suggest the possibility of variation in MSL complex activity among somatic tissues. We hypothesize the degree, up to but not exceeding 2-fold, to which the MSL complex upregulates male X-linked genes varies quantitatively by tissue type. In this model, MSL complex abundance acts as a rheostat to control the extent of upregulation. Using publicly available expression data, we provide evidence for our model in Drosophila somatic tissues. Specifically, we find X-to-autosome expression correlates with the tissue-specific expression of msl-2 which encodes an essential male-specific component of the MSL complex. This result suggests MSL complex mediated dosage compensation varies quantitatively by tissue type. Furthermore, this result has consequences for models explaining the organismal-scale molecular and evolutionary consequences of MSL-mediated dosage compensation.
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DOI：
10.1126/science.1231815
发表时间：
2013-04-19
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ferrari F;Jung YL;Kharchenko PV;Plachetka A;Alekseyenko AA;Kuroda MI;Park PJ
通讯作者：
Park PJ
影响因子：
56.9
作者：
Park, Y;Kelley, RL;Meller, VH
通讯作者：
Meller, VH
影响因子：
64.5
作者：
Alekseyenko AA;Peng S;Larschan E;Gorchakov AA;Lee OK;Kharchenko P;McGrath SD;Wang CI;Mardis ER;Park PJ;Kuroda MI
通讯作者：
Kuroda MI
影响因子：
10.5
作者：
Hamada, FN;Park, PJ;Kuroda, MI
通讯作者：
Kuroda, MI
影响因子：
12.3
作者：
Parisi M;Nuttall R;Edwards P;Minor J;Naiman D;Lü J;Doctolero M;Vainer M;Chan C;Malley J;Eastman S;Oliver B
通讯作者：
Oliver B