A novel and accurate microfluidic assay of CD62L in bladder cancer serum samples.
A novel and accurate microfluidic assay of CD62L in bladder cancer serum samples.
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DOI：
10.1039/c8an01463a
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发表时间：
2018-11-05
期刊：
影响因子：
--
通讯作者：
Rusling JF
中科院分区：
文献类型：
--
作者：
Phadke GS ;Satterwhite-Warden JE ;Choudhary D ;Taylor JA ;Rusling JF
我们报道了一种低成本，敏感，基于珠状的电化学免疫阵列，用于可溶性l -选择素（或CD62L蛋白），一种潜在的膀胱癌分期生物标志物。我们使用半自动模块化微流控阵列，在超顺磁珠上在线捕获抗原，随后将抗原传递到包含多个传感器的检测室。该方法可准确检测稀释后血清中的CD62L，检测限（LOD）为0.25 ng mL−1，动态范围为0.25 ~ 100 ng mL−1。与标准ELISA试剂盒相比，微流体阵列具有更好的准确性和更高的灵敏度，但在高、低浓度范围内存在显著的系统误差。采用免疫阵列法和ELISA法对31例不同级别膀胱癌患者和无癌对照组的血清样本进行分析，发现CD62L水平存在相关性。这项工作建立了一种新的准确测定CD62L水平的方法，并强调了该蛋白作为检测膀胱癌局部区域进展的生物标志物的潜力。基于磁珠的CD62L微流控免疫阵列可以区分低级别和高级别膀胱癌肿瘤。
We report a low-cost, sensitive, bead-based electrochemical immunoarray for soluble L-selectin (or CD62L protein), a potential biomarker for staging bladder cancer. We used a semi-automated modular microfluidic array with online antigen capture on superparamagnetic beads, which were subsequently delivered to a detection chamber housing multiple sensors. The assay was designed to accurately detect CD62L in diluted serum with a limit of detection (LOD) of 0.25 ng mL−1 and a dynamic range of 0.25–100 ng mL−1. The microfluidic array gave significantly better accuracy and higher sensitivity than a standard ELISA kit, which was shown to be subject to significant systematic error at high and low concentration ranges. 31 serum samples from patients with varying grades of bladder cancer and cancer-free controls were analyzed by the immunoarray and ELISA, and the CD62L levels correlated. This work establishes a new accurate assay for determining CD62L levels and highlights the potential of this protein as a biomarker for detecting locoregional progression of bladder cancer. A magnetic bead-based, microfluidic immunoarray of CD62L can distinguish between low- and high-grade bladder cancer tumors.
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影响因子：
12.6
作者：
Chikkaveeraiah, Bhaskara V.;Mani, Vigneshwaran;Patel, Vyomesh;Gutkind, J. Silvio;Rusling, James F.
通讯作者：
Rusling, James F.
影响因子：
17.1
作者：
Mani, Vigneshwaran;Chikkaveeraiah, Bhaskara V.;Patel, Vyomesh;Gutkind, J. Silvio;Rusling, James F.
通讯作者：
Rusling, James F.
影响因子：
20.3
作者：
Bélanger, SD;St-Pierre, Y
通讯作者：
St-Pierre, Y
DOI：
10.1073/pnas.061633698
发表时间：
2001-03-27
影响因子：
11.1
作者：
Qian, F;Hanahan, D;Weissman, IL
通讯作者：
Weissman, IL
影响因子：
4.3
作者：
Herrasti, Zorione;de la Serna, Erica;Baldrich, Eva
通讯作者：
Baldrich, Eva