Transcriptomic assessment of resistance to effects of an aryl hydrocarbon receptor (AHR) agonist in embryos of Atlantic killifish (Fundulus heteroclitus) from a marine Superfund site.
Transcriptomic assessment of resistance to effects of an aryl hydrocarbon receptor (AHR) agonist in embryos of Atlantic killifish (Fundulus heteroclitus) from a marine Superfund site.
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转录组对芳基烃受体（AHR）激动剂在大西洋Killifish（heteroclitus）胚胎中对芳基烃受体（AHR）激动剂的影响的抗性评估。
DOI：
10.1186/1471-2164-12-263
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发表时间：
2011-05-24
期刊：
影响因子：
4.4
通讯作者：
Hahn ME
中科院分区：
文献类型：
--
作者：
Oleksiak MF;Karchner SI;Jenny MJ;Franks DG;Welch DB;Hahn ME
大西洋无须鳕（Fundulus heteroclitus）的种群已经进化出对多氯联苯（PCB）和其他卤代和非卤代芳烃的胚胎毒性效应的抗性，这些效应通过芳烃受体（AHR）依赖的信号通路发挥作用。耐药性伴随着对细胞色素P450 1A（CYP 1A）诱导的敏感性降低，细胞色素P450 1A是一种广泛使用的芳烃暴露和影响的生物标志物，但敏感性降低是否特异于CYP 1A或反映了对所有AHR介导的基因表达变化的反应性的全基因组降低尚不清楚。比较了3，3 '，4，4'，5-五氯联苯（PCB-126）暴露于不同浓度的三疣梭子蟹胚胎（5和10 dpf）和幼虫（15 dpf）中的基因表达谱和对PCB-126暴露的反应。栖息在马萨诸塞州新贝德福德港（NBH）超级基金站点（多氯联苯抗性）和参考站点，马萨诸塞州斯科顿溪（SC;多氯联苯敏感）的异缘蜱种群。使用7，000个基因的cDNA阵列分析显示，人群之间对PCB-126的反应存在显著差异;差异发生在所有三个阶段。有一个相当大的一套PCB-responsive基因在敏感的SC人口，一个小得多的一套PCB-responsive基因在NBH鱼，PCB-responsive基因之间的两个人口之间的相似性很少。通过RNA-Seq（深度焦磷酸测序）确认了大多数阵列结果，并鉴定了其他PCB调控基因。结果表明，NBH鱼具有基因调控缺陷，这不是特定于一个靶基因，如CYP 1A，而是在于一个调控途径，控制多个基因的转录反应PCB曝光。结果与NBH鱼中AHR依赖性信号传导的全基因组破坏一致。
Populations of Atlantic killifish (Fundulus heteroclitus) have evolved resistance to the embryotoxic effects of polychlorinated biphenyls (PCBs) and other halogenated and nonhalogenated aromatic hydrocarbons that act through an aryl hydrocarbon receptor (AHR)-dependent signaling pathway. The resistance is accompanied by reduced sensitivity to induction of cytochrome P450 1A (CYP1A), a widely used biomarker of aromatic hydrocarbon exposure and effect, but whether the reduced sensitivity is specific to CYP1A or reflects a genome-wide reduction in responsiveness to all AHR-mediated changes in gene expression is unknown. We compared gene expression profiles and the response to 3,3',4,4',5-pentachlorobiphenyl (PCB-126) exposure in embryos (5 and 10 dpf) and larvae (15 dpf) from F. heteroclitus populations inhabiting the New Bedford Harbor, Massachusetts (NBH) Superfund site (PCB-resistant) and a reference site, Scorton Creek, Massachusetts (SC; PCB-sensitive). Analysis using a 7,000-gene cDNA array revealed striking differences in responsiveness to PCB-126 between the populations; the differences occur at all three stages examined. There was a sizeable set of PCB-responsive genes in the sensitive SC population, a much smaller set of PCB-responsive genes in NBH fish, and few similarities in PCB-responsive genes between the two populations. Most of the array results were confirmed, and additional PCB-regulated genes identified, by RNA-Seq (deep pyrosequencing). The results suggest that NBH fish possess a gene regulatory defect that is not specific to one target gene such as CYP1A but rather lies in a regulatory pathway that controls the transcriptional response of multiple genes to PCB exposure. The results are consistent with genome-wide disruption of AHR-dependent signaling in NBH fish.
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影响因子：
4.4
作者：
Fisher, Marla A.;Oleksiak, Marjorie F.
通讯作者：
Oleksiak, Marjorie F.
影响因子：
4.5
作者：
Aluru, Neelakanteswar;Karchner, Sibel I.;Hahn, Mark E.
通讯作者：
Hahn, Mark E.
影响因子：
4.4
作者：
Bozinovic, Goran;Sit, Tim L.;Oleksiak, Marjorie F.
通讯作者：
Oleksiak, Marjorie F.
影响因子：
4.8
作者：
Karchner, SI;Powell, WH;Hahn, ME
通讯作者：
Hahn, ME
影响因子：
3.8
作者：
Boutros, Paul C.;Yao, Cindy Q.;Pohjanvirta, Raimo
通讯作者：
Pohjanvirta, Raimo