Evidence that cultured airway smooth muscle cells contain bradykinin B2 and B3 receptors.
Evidence that cultured airway smooth muscle cells contain bradykinin B2 and B3 receptors.
复制标题
有证据表明培养的气道平滑肌细胞含有缓激肽 B2 和 B3 受体。
DOI：
10.1165/ajrcmb/4.3.273
复制
发表时间：
1991
影响因子：
6.4
通讯作者：
Ronald M. Burch
中科院分区：
文献类型：
--
作者：
Stephen G. Farmer;Jeffrey E. Ensor;Ronald M. Burch
在组织培养的豚鼠气管平滑肌细胞中，我们检测了缓激肽诱导的45Ca2+外排和前列腺素合成。我们还研究了B1受体激动剂和拮抗剂、B2受体拮抗剂和环氧合酶抑制剂吲哚美辛的作用。在培养的平滑肌细胞中，缓激素（0.1 nM至10微米）刺激45Ca2+的外排，诱导前列腺素E2和前列腺素代谢物6-酮-前列腺素F1 α的合成。desArg9- bradykinin是一种B1受体激动剂，对45Ca2+外排或前列腺素合成没有影响，而且B1受体拮抗剂desArg9-[Leu8]-bradykinin对减慢激肽的反应也不受其影响。吲哚美辛（1微米）可消除缓激肽诱导的前列腺素合成，但对45Ca2+外排无影响。B2受体拮抗剂NPC 567 （DArg[Hyp3，DPhe7]-缓激肽）对缓激肽诱导的45Ca2+外排无影响，但能抑制前列腺素的合成。与新鲜制备的豚鼠气管平滑肌膜不同，B2受体拮抗剂完全抑制[3H]-缓激肽与培养气管平滑肌细胞膜的结合（Ki, 12 nM）。这些数据表明，在培养中维持的气管平滑肌细胞表达B2受体，介导缓激肽诱导的前列腺素合成。缓激素诱导的钙离子外排不受B1或B2拮抗剂的影响，这一观察结果进一步证明气道平滑肌可能含有一种新的B3受体。
We examined bradykinin-induced 45Ca2+ efflux and prostaglandin synthesis in guinea pig tracheal smooth muscle cells maintained in tissue culture. We also studied the effects of a B1 receptor agonist and antagonist, a B2 receptor antagonist, and the cyclooxygenase inhibitor indomethacin. In cultured smooth muscle cells, bradykinin (0.1 nM to 10 microM) stimulated efflux of 45Ca2+ and induced the synthesis of prostaglandin E2 and the prostacyclin metabolite 6-keto-prostaglandin F1 alpha. DesArg9-bradykinin, a B1 receptor agonist, had no effect on 45Ca2+ efflux or prostaglandin synthesis, and no responses to bradykinin were unaffected by the B1 receptor antagonist desArg9-[Leu8]-bradykinin. Indomethacin (1 microM) abolished bradykinin-induced prostaglandin synthesis but was without effect on 45Ca2+ efflux. NPC 567 (DArg[Hyp3,DPhe7]-bradykinin), a B2 receptor antagonist, had no effect on bradykinin-induced 45Ca2+ efflux, but abolished prostaglandin synthesis. Unlike in membranes prepared freshly from guinea pig tracheal smooth muscle, the B2 receptor antagonist inhibited completely (Ki, 12 nM) binding of [3H]-bradykinin to membranes prepared from cultured tracheal smooth cells. These data suggest that tracheal smooth muscle cells, maintained in culture, express B2 receptors that mediate bradykinin-induced prostaglandin synthesis. The observation that bradykinin-induced efflux of calcium ions was unaffected by B1 or B2 antagonists provides further evidence that airway smooth muscle may contain a novel B3 receptor.
影响因子：
--
作者：
PANETTIERI, RA;MURRAY, RK;KOTLIKOFF, MI
通讯作者：
KOTLIKOFF, MI
影响因子：
56.9
作者：
ORDWAY, RW;WALSH, JV;SINGER, JJ
通讯作者：
SINGER, JJ
细胞培养中的人气道平滑肌：细胞内钙储存的控制。
DOI：
10.1016/s0952-0600(89)80009-2
发表时间：
1989
期刊：
Pulmonary pharmacology
影响因子：
--
作者：
Twort,CH;vanBreemen,C
通讯作者：
vanBreemen,C