In vitro and in vivo anti-colon cancer effects of Garcinia mangostana xanthones extract.
In vitro and in vivo anti-colon cancer effects of Garcinia mangostana xanthones extract.
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DOI：
10.1186/1472-6882-12-104
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发表时间：
2012-07-20
影响因子：
--
通讯作者：
Majid AM
中科院分区：
文献类型：
--
作者：
Aisha AF;Abu-Salah KM;Ismail Z;Majid AM
氧杂蒽酮是一类具有抗癌、抗氧化、抗炎、抗菌等药理作用的含氧杂环化合物。通过结晶G.的甲苯提取物来制备氧杂蒽酮提取物（81% α-倒果木黄酮和16% γ-倒果木黄酮）。采用HCT 116人结肠癌细胞系研究了芒果果皮提取物的抗结肠癌作用，包括细胞毒性、细胞凋亡、抗肿瘤作用以及对细胞信号通路的影响。还在裸鼠中建立的皮下肿瘤上研究了体内抗结肠癌活性。由于诱导线粒体凋亡途径，浸提液显示出强效细胞毒性（中位抑制浓度6.5 ± 1.0 μg/ml）。肿瘤转移的三个关键步骤，即细胞迁移、细胞侵袭和克隆形成也受到抑制。提取物和α-mangostin上调MAPK/ERK、c-Myc/Max和p53细胞信号通路。口山酮提取物，当喂给裸鼠时，引起HCT 116结肠直肠癌细胞皮下肿瘤的显著生长抑制。我们的数据提示了α-mangostin和G. mangostana xanthones，并建议xanthones提取物作为一个潜在的抗结肠癌的候选。
Xanthones are a group of oxygen-containing heterocyclic compounds with remarkable pharmacological effects such as anti-cancer, antioxidant, anti-inflammatory, and antimicrobial activities. A xanthones extract (81% α-mangostin and 16% γ-mangostin), was prepared by crystallization of a toluene extract of G. mangostana fruit rinds and was analyzed by LC-MS. Anti-colon cancer effect was investigated on HCT 116 human colorectal carcinoma cells including cytotoxicity, apoptosis, anti-tumorigenicity, and effect on cell signalling pathways. The in vivo anti-colon cancer activity was also investigated on subcutaneous tumors established in nude mice. The extract showed potent cytotoxicity (median inhibitory concentration 6.5 ± 1.0 μg/ml), due to induction of the mitochondrial pathway of apoptosis. Three key steps in tumor metastasis including the cell migration, cell invasion and clonogenicity, were also inhibited. The extract and α-mangostin up-regulate the MAPK/ERK, c-Myc/Max, and p53 cell signalling pathways. The xanthones extract, when fed to nude mice, caused significant growth inhibition of the subcutaneous tumor of HCT 116 colorectal carcinoma cells. Our data suggest new mechanisms of action of α-mangostin and the G. mangostana xanthones, and suggest the xanthones extract of as a potential anti-colon cancer candidate.
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影响因子：
6.1
作者：
Jung, HA;Su, BN;Kinghorn, AD
通讯作者：
Kinghorn, AD
影响因子：
2
作者：
Nakatani, K;Atsumi, M;Ohizumi, Y
通讯作者：
Ohizumi, Y
DOI：
10.3923/ijcr.2009.105.114
发表时间：
2009-01-01
期刊：
International Journal of Cancer Research (USA)
影响因子：
--
作者：
Aisha, A. F. A.;Sahib, H. B.;Abdul Majid, A. M. S.
通讯作者：
Abdul Majid, A. M. S.
影响因子：
8
作者：
Hardwick, JCH;van den Brink, GR;Peppelenbosch, MP
通讯作者：
Peppelenbosch, MP
DOI：
10.1073/pnas.77.2.990
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
JOHNSON, LV;WALSH, ML;CHEN, LB
通讯作者：
CHEN, LB