Multi-locus sequence typing of a geographically and temporally diverse sample of the highly clonal human pathogen Bartonella quintana.
Multi-locus sequence typing of a geographically and temporally diverse sample of the highly clonal human pathogen Bartonella quintana.
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DOI：
10.1371/journal.pone.0009765
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发表时间：
2010-03-19
期刊：
影响因子：
3.7
通讯作者：
Feil EJ
中科院分区：
文献类型：
--
作者：
Arvand M;Raoult D;Feil EJ
金塔那巴尔通体是一种重新出现的病原体，是人类多种疾病表现的病原体，包括战壕热。针对B. quintana已经开发了各种类型方法，但这些方法往往受到分辨率差的限制，并且在基于凝胶的方法中，缺乏可移植性。多位点序列分型（MLST）已被用于研究大量病原体的分子流行病学，包括B. henselae， B. quintana的近亲。我们开发了一种基于7个MLST位点的昆塔纳白蝇的MLST方案，其中两个额外的位点覆盖了昆塔纳白蝇染色体上未被充分代表的区域。共有16株菌株分布在3个大洲，历时60年。9个位点中有5个检测到等位基因变异。虽然只有8/4270（0.002%）的核苷酸位点在所有位点上都是可变的，但这些多态性将16株分离株划分为7种序列类型（STs）。我们还证明了MLST可以通过直接PCR应用于心脏瓣膜组织的非培养分离物，并表明该方法为这种高度克隆生物的流行病学研究提供了一种很有前途的方法。系统发育和聚类分析表明，7个STs中的2个在种群中形成了一个不同的谱系。
Bartonella quintana is a re-emerging pathogen and the causative agent of a variety of disease manifestations in humans including trench fever. Various typing methods have been developed for B. quintana, but these tend to be limited by poor resolution and, in the case of gel-based methods, a lack of portability. Multilocus sequence typing (MLST) has been used to study the molecular epidemiology of a large number of pathogens, including B. henselae, a close relative of B. quintana. We developed a MLST scheme for B. quintana based on the 7 MLST loci employed for B. henselae with two additional loci to cover underrepresented regions of the B. quintana chromosome. A total of 16 B. quintana isolates spanning over 60 years and three continents were characterized. Allelic variation was detected in five of the nine loci. Although only 8/4270 (0.002%) of the nucleotide sites examined were variable over all loci, these polymorphisms resolved the 16 isolates into seven sequence types (STs). We also demonstrate that MLST can be applied on uncultured isolates by direct PCR from cardiac valve tissue, and suggest this method presents a promising approach for epidemiological studies in this highly clonal organism. Phylogenetic and clustering analyses suggest that two of the seven STs form a distinct lineage within the population.
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影响因子：
11.8
作者：
O'Rourke LG;Pitulle C;Hegarty BC;Kraycirik S;Killary KA;Grosenstein P;Brown JW;Breitschwerdt EB
通讯作者：
Breitschwerdt EB
DOI：
10.1099/00207713-49-3-1015
发表时间：
1999-07-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
Marston, EL;Sumner, JW;Regnery, RL
通讯作者：
Regnery, RL
影响因子：
9.4
作者：
MAURIN, M;ROUX, V;RAOULT, D
通讯作者：
RAOULT, D
影响因子：
9.4
作者：
ROUX, V;RAOULT, D
通讯作者：
RAOULT, D
影响因子：
9.4
作者：
Homan, WL;Tribe, D;Willems, RJL
通讯作者：
Willems, RJL