Morphological changes to Schlemm's canal and the distal aqueous outflow pathway in monkey eyes with laser-induced ocular hypertension.
Morphological changes to Schlemm's canal and the distal aqueous outflow pathway in monkey eyes with laser-induced ocular hypertension.
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DOI：
10.1016/j.exer.2022.109030
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发表时间：
2022-06
影响因子：
3.4
通讯作者：
Gong, Haiyan
中科院分区：
文献类型：
--
作者：
Sosnowik, Shayna;Swain, David L. L.;Fan, Shan;Toris, Carol B. B.;Gong, Haiyan
关键词：
虽然大约 30-50% 的房水流出阻力位于施累姆氏管 (SC) 远端，但 SC 远端传统流出路径的形态尚未得到彻底评估。本研究检查了食蟹猴青光眼实验模型中传统房水流出路径近端和远端的形态变化及其与流出设施减少的关系。 Nd:YAG 激光烧伤每只猴子的一只眼睛 (n = 6) 或双眼 (n = 2) 的小梁网 (TM) 270-340 度，以诱发高眼压症。 TM 的不同区域未受到激光照射。对侧眼睛 (n = 5) 未接受激光照射并用作对照。猴子在最后一次激光治疗后 ≥ 60 个月被处死。所有眼睛均被摘除并在 15 mmHg 下灌注 30 分钟以测量流出设施。两双眼睛也灌注荧光素以检查节段流出。所有眼睛均进行灌注固定1小时。将前节切成放射状楔形并进行光学和电子显微镜检查。比较对照眼的高流量区域和低流量区域以及激光治疗眼和对照眼的非激光区域之间的 SC 宽度、高度和横截面积 (CSA)。比较激光治疗眼和对照眼的非激光和激光区域之间以及对照眼的高流量和低流量区域之间的巩膜内静脉（ISV）的数量和CSA。比较对照眼与激光治疗眼的激光和非激光区域之间的巩膜胶原纤维直径。与对照眼相比，激光治疗眼的中位流出设施显着降低（P = 0.02）。对照眼的高流量区域的中位 CSA 和 SC 高度小于低流量区域（P < 0.05）。对照眼的高流量区域和低流量区域之间 SC 的中位宽度没有显着差异（P > 0.05）。非激光区域和对照眼之间的中位 CSA、SC 宽度和高度没有差异 (P > 0.05)。 SC 在激光照射区域被部分或完全消除。与非激光区域 (P < 0.01) 和对照眼 (P < 0.01) 相比，激光区域的 ISV 中位数显着减少。 ISV 的中位 CSA 在这些组之间没有差异 (P > 0.05)。对照眼的高流量区域和低流量区域之间 ISV 的中位数和 CSA 没有显着差异（P > 0.05）。与非激光区域 (P < 0.05) 和对照眼 (P < 0.05) 相比，激光区域显示出更疏松的巩膜基质和邻近 TM 的胶原原纤维的中值直径更小。密集的TM、SC的部分或完全消失以及专利ISV数量的减少可能部分解释了激光诱导高眼压的猴眼流出能力的减少。激光治疗眼睛巩膜结构变化的意义值得进一步研究。
Though roughly 30–50% of aqueous outflow resistance resides distal to Schlemm’s canal (SC), the morphology of the conventional outflow pathway distal to SC has not been thoroughly evaluated. This study examined the morphological changes along proximal and distal aspects of the conventional aqueous outflow pathway and their association with decreased outflow facility in an experimental model of glaucoma in cynomolgus macaques. Nd:YAG laser burns were made to 270–340 degrees of the trabecular meshwork (TM) of one eye (n = 6) or both eyes (n = 2) of each monkey to induce ocular hypertension. Distinct regions of the TM were left unlasered. Contralateral eyes (n = 5) were not lasered and were utilized as controls. Monkeys were sacrificed ≥ 60 months after their last laser treatment. All eyes were enucleated and perfused at 15 mmHg for 30 minutes to measure outflow facility. Two pairs of eyes were also perfused with fluorescein to examine segmental outflow. All eyes underwent perfusion-fixation for 1 hr. Anterior segments were cut into radial wedges and processed for light and electron microscopy. Width, height, and cross-sectional area (CSA) of SC were compared between high- and low-flow regions of control eyes, and between non-lasered regions of laser-treated eyes and control eyes. Number and CSA of intrascleral veins (ISVs) were compared between non-lasered and lasered regions of laser-treated eyes and control eyes, and between high- and low-flow regions of control eyes. Scleral collagen fibril diameter was compared between control eyes and lasered and non-lasered regions of laser-treated eyes. Median outflow facility was significantly decreased in laser-treated eyes compared to control eyes (P = 0.02). Median CSA and height of SC were smaller in high- flow regions than low-flow regions of control eyes (P < 0.05). Median width of SC was not significantly different between high- and low-flow regions of control eyes (P > 0.05). Median CSA, width, and height of SC were not different between non-lasered regions and control eyes (P > 0.05). SC was partially or completely obliterated in lasered regions. Median number of ISVs was significantly decreased in lasered regions compared to non-lasered regions (P < 0.01) and control eyes (P < 0.01). Median CSA of ISVs did not differ between these groups (P > 0.05). Median number and CSA of ISVs were not significantly different between high- and low-flow regions of control eyes (P > 0.05). Lasered regions displayed looser scleral stroma and smaller median diameter of collagen fibrils adjacent to the TM compared to non-lasered regions (P < 0.05) and control eyes (P < 0.05). Dense TM, partial to complete obliteration of SC, and a decreased number of patent ISVs may account in part for the decreased outflow facility in monkey eyes with laser-induced ocular hypertension. The significance of changes in scleral structure in laser-treated eyes warrants further investigation.
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影响因子：
3.4
作者：
BILL, A
通讯作者：
BILL, A
影响因子：
4.4
作者：
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作者：
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影响因子：
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作者：
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