The relationship between environmental exposures to phthalates and DNA damage in human sperm using the neutral comet assay.
The relationship between environmental exposures to phthalates and DNA damage in human sperm using the neutral comet assay.
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DOI：
10.1289/ehp.5756
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发表时间：
2003-07
影响因子：
10.4
通讯作者：
Hauser R
中科院分区：
文献类型：
--
作者：
Duty SM;Singh NP;Silva MJ;Barr DB;Brock JW;Ryan L;Herrick RF;Christiani DC;Hauser R
邻苯二甲酸酯是在许多商业应用中广泛使用的工业化学品。普通人群通过消费品以及饮食和医疗接触邻苯二甲酸酯。为了确定邻苯二甲酸酯的环境水平是否与人类精子中DNA完整性的改变有关，我们选择了一个没有确定邻苯二甲酸酯暴露源的人群。从马萨诸塞州总医院男科实验室招募的168名受试者提供了精液和尿液样本。使用高效液相色谱和串联质谱法测定尿液中的8种邻苯二甲酸酯代谢物;通过调整比重校正尿液稀释数据。中性单细胞微凝胶电泳法（彗星试验）用于测量精子中DNA的完整性。VisComet图像分析软件用于测量彗星范围，即总彗星长度（微米）的量度;尾中DNA百分比（尾%），即存在于彗星尾中的总DNA的比例的量度;以及尾分布矩（TDM），即长度和强度（微米）的综合量度。对于比重调整的邻苯二甲酸单乙酯（MEP）水平的四分位距增加，彗星范围显著增加3.6 μ m [95%置信区间（95% CI），0.74-6.47]; TDM也增加1.2 μ m（95% CI，-0.05至2.38），但具有临界显著性。邻苯二甲酸单丁酯、邻苯二甲酸单苄酯、邻苯二甲酸单甲酯和邻苯二甲酸单-2-乙基己酯与彗星试验参数无显著相关性。总之，这项研究代表了第一个人类数据，证明在环境水平上，尿MEP与精子中DNA损伤增加有关。
Phthalates are industrial chemicals widely used in many commercial applications. The general population is exposed to phthalates through consumer products as well as through diet and medical treatments. To determine whether environmental levels of phthalates are associated with altered DNA integrity in human sperm, we selected a population without identified sources of exposure to phthalates. One hundred sixty-eight subjects recruited from the Massachusetts General Hospital Andrology Laboratory provided a semen and a urine sample. Eight phthalate metabolites were measured in urine by using high-performance liquid chromatography and tandem mass spectrometry; data were corrected for urine dilution by adjusting for specific gravity. The neutral single-cell microgel electrophoresis assay (comet assay) was used to measure DNA integrity in sperm. VisComet image analysis software was used to measure comet extent, a measure of total comet length (micrometers); percent DNA in tail (tail%), a measure of the proportion of total DNA present in the comet tail; and tail distributed moment (TDM), an integrated measure of length and intensity (micrometers). For an interquartile range increase in specific gravity-adjusted monoethyl phthalate (MEP) level, the comet extent increased significantly by 3.6 micro m [95% confidence interval (95% CI), 0.74-6.47]; the TDM also increased 1.2 micro m (95% CI, -0.05 to 2.38) but was of borderline significance. Monobutyl, monobenzyl, monomethyl, and mono-2-ethylhexyl phthalates were not significantly associated with comet assay parameters. In conclusion, this study represents the first human data to demonstrate that urinary MEP, at environmental levels, is associated with increased DNA damage in sperm.
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影响因子：
10.4
作者：
GANGOLLI, SD
通讯作者：
GANGOLLI, SD
DOI：
10.1002/tcm.1007
发表时间：
2001-01-01
期刊：
TERATOGENESIS CARCINOGENESIS AND MUTAGENESIS
影响因子：
--
作者：
Kleinsasser, NH;Wallner, BC;Harréus, UA
通讯作者：
Harréus, UA
影响因子：
7.4
作者：
Blount, BC;Milgram, KE;Brock, JW
通讯作者：
Brock, JW
影响因子：
10.4
作者：
Koo, JW;Parham, F;Portier, CJ
通讯作者：
Portier, CJ
影响因子：
10.4
作者：
Lahdetie, J;AjosenpaaSaari, M;Mykkanen, J
通讯作者：
Mykkanen, J