Evaluating the Distribution of Bacterial Natural Product Biosynthetic Genes across Lake Huron Sediment.
Evaluating the Distribution of Bacterial Natural Product Biosynthetic Genes across Lake Huron Sediment.
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DOI：
10.1021/acschembio.1c00653
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发表时间：
2021-11-19
影响因子：
4
通讯作者：
Murphy BT
中科院分区：
文献类型：
--
作者：
Elfeki M;Mantri S;Clark CM;Green SJ;Ziemert N;Murphy BT
环境微生物继续作为生物活性天然产物（NP）的主要来源，并作为现代医学工具箱中许多其他支架的灵感来源。几乎所有的微生物NP启发疗法都可以追溯到从环境中收集样本的实地考察。尽管这些探险在寻找新药方面很重要，但很少有研究试图记录NP或其相应的生产基因在给定环境中的分布程度。为了深入了解这一点，NP酮合酶（KS）和腺苷酸化（A）域的地理发生分别记录在53和58个表面沉积物样本，覆盖休伦湖59,590平方公里。总体而言，在调查中检测到的90，528种NP类非核糖体肽和聚酮化合物中没有观察到可辨别的NP地理分布模式。虽然每个采样位置具有相似数量的A结构域操作生物合成单位（OBU），但观察到OBU类型的有限重叠，表明在本研究中使用的测序深度，没有单一位置作为NP“热点”。这些数据支持的假设，有足够的变化，在NP发生采样点之间，并建议，广泛的样品收集工作，需要充分捕捉功能的化学多样性沉积物微生物群落在区域范围内。
Environmental microorganisms continue to serve as a major source of bioactive natural products (NPs) and as an inspiration for many other scaffolds in the toolbox of modern medicine. Nearly all microbial NP-inspired therapies can be traced to field expeditions to collect samples from the environment. Despite the importance of these expeditions in the search for new drugs, few studies have attempted to document the extent to which NPs or their corresponding production genes are distributed within a given environment. To gain insight into this, the geographic occurrence of NP ketosynthase (KS) and adenylation (A) domains was documented across 53 and 58 surface sediment samples, respectively, covering 59,590 square kilometers of Lake Huron. Overall, no discernable NP geographic distribution patterns were observed for 90,528 NP classes of nonribosomal peptides and polyketides detected in the survey. While each sampling location harbored a similar number of A domain operational biosynthetic units (OBUs), limited overlap of OBU type was observed, suggesting that at the sequencing depth used in this study, no single location served as a NP ‘hotspot’. These data support the hypothesis that there is ample variation in NP occurrence between sampling sites and suggests that extensive sample collection efforts are required to fully capture the functional chemical diversity of sediment microbial communities on a regional scale.
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影响因子：
14.9
作者：
Abascal F;Zardoya R;Telford MJ
通讯作者：
Telford MJ
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
DOI：
10.1126/science.1176667
发表时间：
2009-08-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Fischbach MA;Walsh CT
通讯作者：
Walsh CT
白黄链霉菌的群体遗传分析揭示了链霉菌多样化中同源重组的栖息地障碍
影响因子：
4.4
作者：
Cheng, Kun;Rong, Xiaoying;Huang, Ying
通讯作者：
Huang, Ying
影响因子：
64.8
作者：
Bentley, SD;Chater, KF;Hopwood, DA
通讯作者：
Hopwood, DA