The Arabidopsis cytochrome P450 CYP86A1 encodes a fatty acid omega-hydroxylase involved in suberin monomer biosynthesis.
The Arabidopsis cytochrome P450 CYP86A1 encodes a fatty acid omega-hydroxylase involved in suberin monomer biosynthesis.
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拟南芥细胞色素P450 CYP86A1编码参与suberin单体生物合成的脂肪酸欧米茄羟化酶。
DOI：
10.1093/jxb/ern101
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发表时间：
2008
影响因子：
6.9
通讯作者：
Franke R
中科院分区：
文献类型：
--
作者：
Höfer R;Briesen I;Beck M;Pinot F;Schreiber L;Franke R
亲脂性生物聚合酯Suberin形成重要的边界，以保护植物与其周围环境或分离植物内的不同组织。在根中，Suberin存在于内胚层和皮下层或周层的细胞壁中。由Suberin组成的质外体屏障完成了限制水分和养分流失和防止病原体入侵的挑战。尽管Suberin具有重要的生理意义，也知道许多植物的Suberin组成，但人们对其生物合成和相关基因知之甚少。在这里，详细分析了拟南芥不同发育阶段根中脂肪族Suberin的含量。这项研究证明了大豆黄素的数量和组成在根轴上存在一定的变异性，并表明了ω羟化对大豆黄素生物合成的重要性。利用反向遗传学方法，细胞色素P450脂肪酸ω-羟基酶CYP86A1(AT5g58860)已被鉴定为拟南芥脂肪根山梨素生物合成的关键酶。相应的Horst突变体显示ω-羟基酸显著减少，其链长为lt；C20，这表明该突变体作为一种根系硬脂化组织的羟基酶发挥作用。详细的表达研究表明，它具有很强的根部特异性，并且在根部内皮层有局部表达。融合了绿色荧光蛋白的细胞色素P86A1的转基因表达将细胞色素P86A1分布到内质网中，这表明Suberin单体的生物合成发生在这个亚细胞室，中间产物在质外体输出之前。
The lipophilic biopolyester suberin forms important boundaries to protect the plant from its surrounding environment or to separate different tissues within the plant. In roots, suberin can be found in the cell walls of the endodermis and the hypodermis or periderm. Apoplastic barriers composed of suberin accomplish the challenge to restrict water and nutrient loss and prevent the invasion of pathogens. Despite the physiological importance of suberin and the knowledge of the suberin composition of many plants, very little is known about its biosynthesis and the genes involved. Here, a detailed analysis of the Arabidopsis aliphatic suberin in roots at different developmental stages is presented. This study demonstrates some variability in suberin amount and composition along the root axis and indicates the importance of ω-hydroxylation for suberin biosynthesis. Using reverse genetics, the cytochrome P450 fatty acid ω-hydroxylase CYP86A1 (At5g58860) has been identified as a key enzyme for aliphatic root suberin biosynthesis in Arabidopsis. The corresponding horst mutants show a substantial reduction in ω-hydroxyacids with a chain length <C20, demonstrating that CYP86A1 functions as a hydroxylase of root suberized tissue. Detailed expression studies revealed a strong root specificity and a localized expression in the root endodermis. Transgenic expression of CYP86A1 fused to GFP distributed CYP86A1 to the endoplasmic reticulum, indicating that suberin monomer biosynthesis takes place in this sub-cellular compartment before intermediates are exported in the apoplast.
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影响因子：
7.7
作者：
Kandel, S.;Sauveplane, V.;Pinot, F.
通讯作者：
Pinot, F.
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