Evidence of key tinnitus-related brain regions documented by a unique combination of manganese-enhanced MRI and acoustic startle reflex testing.
Evidence of key tinnitus-related brain regions documented by a unique combination of manganese-enhanced MRI and acoustic startle reflex testing.
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DOI：
10.1371/journal.pone.0014260
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发表时间：
2010-12-15
期刊：
影响因子：
3.7
通讯作者：
Berkowitz B
中科院分区：
文献类型：
--
作者：
Holt AG;Bissig D;Mirza N;Rajah G;Berkowitz B
动物模型继续提高我们对耳鸣发病机制的理解，并有助于开发新的治疗方法。然而，在清醒、自由活动的动物中，还没有耳鸣相关病理生理学的诊断生物标志物。为了解决这一差异，我们结合了两种互补的方法来检验可靠的耳鸣模型（大鼠反复服用水杨酸或暴露于单一噪音事件）：抑制声惊吓和锰增强MRI。与噪声耳鸣相比，水杨酸诱发的耳鸣导致广泛的锰摄取异常。两种模型在听觉皮层上都显示出显著差异。只有在下丘背皮质（DCIC），两种模型均表现出异常摄取。因此，DCIC异常的膜去极化似乎在耳鸣介导的活动中很重要。我们的研究结果为进一步研究耳鸣的严重程度和寿命与听力损失和特定脑区神经元活动之间的关系提供了基础，并为跨范式评估治疗效果提供了工具。
Animal models continue to improve our understanding of tinnitus pathogenesis and aid in development of new treatments. However, there are no diagnostic biomarkers for tinnitus-related pathophysiology for use in awake, freely moving animals. To address this disparity, two complementary methods were combined to examine reliable tinnitus models (rats repeatedly administered salicylate or exposed to a single noise event): inhibition of acoustic startle and manganese-enhanced MRI. Salicylate-induced tinnitus resulted in wide spread supernormal manganese uptake compared to noise-induced tinnitus. Neither model demonstrated significant differences in the auditory cortex. Only in the dorsal cortex of the inferior colliculus (DCIC) did both models exhibit supernormal uptake. Therefore, abnormal membrane depolarization in the DCIC appears to be important in tinnitus-mediated activity. Our results provide the foundation for future studies correlating the severity and longevity of tinnitus with hearing loss and neuronal activity in specific brain regions and tools for evaluating treatment efficacy across paradigms.
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DOI：
10.1007/s101620010030
发表时间：
2001-03-01
影响因子：
2.4
作者：
Bauer, CA;Brozoski, TJ
通讯作者：
Brozoski, TJ
影响因子：
2.9
作者：
DIAMOND, IT;JONES, EG;POWELL, TPS
通讯作者：
POWELL, TPS
影响因子：
1.4
作者：
Denk, DM;Heinzl, H;Ehrenberger, K
通讯作者：
Ehrenberger, K
影响因子：
4.4
作者：
Berkowitz, Bruce A.;Roberts, Robin;Luan, Hongmei
通讯作者：
Luan, Hongmei
影响因子：
4.4
作者：
Berkowitz, Bruce A.;Roberts, Robin;Gradianu, Marius
通讯作者：
Gradianu, Marius