Small Regulatory RNAs of Rickettsia conorii.
Small Regulatory RNAs of Rickettsia conorii.
复制标题
DOI：
10.1038/srep36728
复制
发表时间：
2016-11-11
影响因子：
4.6
通讯作者：
Sahni SK
中科院分区：
文献类型：
--
作者：
Narra HP;Schroeder CL;Sahni A;Rojas M;Khanipov K;Fofanov Y;Sahni SK
小的调控RNA是细菌基因表达的重要调节因子，但对它们在立克次体中的流行和功能知之甚少。康诺里丝虫是地中海斑点热的病原体，主要感染人类的微血管内皮细胞，是一种由壁虱传播的病原体。我们通过链特异RNA测序确定了孔氏杆菌在感染人微血管内皮细胞(HMECs)过程中的转录图谱，鉴定了4个核糖开关，13个反式(基因间)和22个顺式(反义)小RNA(称为‘RC_sR’s)。进一步通过Northern杂交证实了4个新的反式作用sRNAs(rCsR31、rCsr33、rCsr35和rCsR42)和已知细菌sRNAs(6S、rNasePbact_a、ff和α-tmRNAs)的独立表达。HMECs感染HMECs后，Rc_sR35和Rc_sR42的表达水平显著升高，而Rc_sR31和Rc_sR33的表达水平相近。我们进一步预测了参与所有重要生物学过程的502个基因作为RC_SRS的潜在靶点，并验证了RC_sR42与CydA(细胞色素D泛喹酚氧化酶亚基I)的相互作用。我们的发现首次证明了康诺里乳杆菌存在转录后核转录后调控机制，以及一种新的RC_sR与其靶mRNA之间的相互作用。
Small regulatory RNAs comprise critically important modulators of gene expression in bacteria, yet very little is known about their prevalence and functions in Rickettsia species. R. conorii, the causative agent of Mediterranean spotted fever, is a tick-borne pathogen that primarily infects microvascular endothelium in humans. We have determined the transcriptional landscape of R. conorii during infection of Human Microvascular Endothelial Cells (HMECs) by strand-specific RNA sequencing to identify 4 riboswitches, 13 trans-acting (intergenic), and 22 cis-acting (antisense) small RNAs (termed ‘Rc_sR’s). Independent expression of four novel trans-acting sRNAs (Rc_sR31, Rc_sR33, Rc_sR35, and Rc_sR42) and known bacterial sRNAs (6S, RNaseP_bact_a, ffs, and α-tmRNA) was next confirmed by Northern hybridization. Comparative analysis during infection of HMECs vis-à-vis tick AAE2 cells revealed significantly higher expression of Rc_sR35 and Rc_sR42 in HMECs, whereas Rc_sR31 and Rc_sR33 were expressed at similar levels in both cell types. We further predicted a total of 502 genes involved in all important biological processes as potential targets of Rc_sRs and validated the interaction of Rc_sR42 with cydA (cytochrome d ubiquinol oxidase subunit I). Our findings constitute the first evidence of the existence of post-transcriptional riboregulatory mechanisms in R. conorii and interactions between a novel Rc_sR and its target mRNA.
登录
查看更多内容
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
3.7
作者：
Gillespie, Joseph J.;Williams, Kelly;Shukla, Maulik;Snyder, Eric E.;Nordberg, Eric K.;Ceraul, Shane M.;Dharmanolla, Chitti;Rainey, Daphne;Soneja, Jeetendra;Shallom, Joshua M.;Vishnubhat, Nataraj Dongre;Wattam, Rebecca;Purkayastha, Anjan;Czar, Michael;Crasta, Oswald;Setubal, Joao C.;Azad, Abdu F.;Sobral, Bruno S.
通讯作者：
Sobral, Bruno S.
影响因子：
5.2
作者：
Faucher SP;Shuman HA
通讯作者：
Shuman HA
影响因子：
3.7
作者：
Gillespie JJ;Ammerman NC;Dreher-Lesnick SM;Rahman MS;Worley MJ;Setubal JC;Sobral BS;Azad AF
通讯作者：
Azad AF
DOI：
10.1126/science.aad9822
发表时间：
2016-04-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dar D;Shamir M;Mellin JR;Koutero M;Stern-Ginossar N;Cossart P;Sorek R
通讯作者：
Sorek R