Long-term multimodal imaging of tumor draining sentinel lymph nodes using mesoporous silica-based nanoprobes.
Long-term multimodal imaging of tumor draining sentinel lymph nodes using mesoporous silica-based nanoprobes.
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DOI：
10.1016/j.biomaterials.2012.02.060
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发表时间：
2012-06
期刊：
影响因子：
14
通讯作者：
Chen, Xiaoyuan
中科院分区：
文献类型：
--
作者：
Huang, Xinglu;Zhang, Fan;Lee, Seulki;Swierczewska, Magdalena;Kiesewetter, Dale O.;Lang, Lixin;Zhang, Guofeng;Zhu, Lei;Gao, Haokao;Choi, Hak Soo;Niu, Gang;Chen, Xiaoyuan
关键词：
前哨淋巴结（sentinel lymph nodes，SLNs）是肿瘤转移的第一道防线，其显像是临床无创追踪肿瘤转移的重要手段。在这项研究中，我们报告了基于介孔二氧化硅的三模态纳米探针的开发和应用，这些纳米探针集成了多个功能部分，以促进近红外光学、磁共振（MR）和正电子发射断层扫描（PET）成像。纳米探针包埋近红外染料ZW 800后，通过螯合反应标记T1造影剂Gd 3+和放射性核素64 Cu。通过体外表征证实了纳米探针的高稳定性和长细胞内保留时间，这有利于长期体内成像。随后在4T1肿瘤转移模型中实现纵向多模态成像以可视化肿瘤引流SLN长达3周。观察到转移性和对侧前哨淋巴结之间的摄取率、颗粒量和对比度存在明显差异。这些发现为设计用于前哨淋巴结定位和肿瘤转移诊断的稳健多功能纳米材料提供了非常有帮助的指导。
The imaging of sentinel lymph nodes (SLNs), the first defense against primary tumor metastasis, has been considered as an important strategy for noninvasive tracking tumor metastasis in clinics. In this study, we report the development and application of mesoporous silica-based triple-modal nanoprobes that integrate multiple functional moieties to facilitate near-infrared optical, magnetic resonance (MR) and positron emission tomography (PET) imaging. After embedding near-infrared dye ZW800, the nanoprobe was labeled with T1 contrast agent Gd3+ and radionuclide 64Cu through chelating reactions. High stability and long intracellular retention time of the nanoprobes was confirmed by in vitro characterization, which facilitate long-term in vivo imaging. Longitudinal multimodal imaging was subsequently achieved to visualize tumor draining SLNs up to 3 weeks in a 4T1 tumor metastatic model. Obvious differences in uptake rate, amount of particles, and contrast between metastatic and contralateral sentinel lymph nodes were observed. These findings provide very helpful guidance for the design of robust multifunctional nanomaterials in SLNs’ mapping and tumor metastasis diagnosis.
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DOI：
10.1007/s00259-009-1158-1
发表时间：
2009-09
影响因子：
9.1
作者：
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影响因子：
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影响因子：
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