Galloyl moieties enhance the dentin biomodification potential of plant-derived catechins.
Galloyl moieties enhance the dentin biomodification potential of plant-derived catechins.
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DOI：
10.1016/j.actbio.2014.03.036
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发表时间：
2014-07
影响因子：
9.7
通讯作者：
Bedran-Russo, Ana
中科院分区：
文献类型：
--
作者：
Vidal, Cristina M. P.;Aguiar, Thaiane R.;Phansalkar, Rasika;McAlpine, James B.;Napolitano, Jose G.;Chen, Shao-Nong;Araujo, Larissa S. N.;Pauli, Guido F.;Bedran-Russo, Ana
Proanthocyanidin (PAC) rich plant-derived agents have been shown to enhance dentin biomechanical properties and resistance to collagenase degradation. This study systematically investigated the interaction of chemically well-defined monomeric catechins with dentin extracellular matrix components by evaluating dentin mechanical properties as well as activities of matrix metalloproteinases (MMPs) and cysteine-cathepsins (CTs). Demineralized dentin beams (n = 15) were incubated for 1 hr with 0.65% (+)-catechin (C), (−)-catechin gallate (CG), (−)-gallocatechin gallate (GCG), (−)-epicatechin (EC), (−)-epicatechin gallate (ECG), (−)-epigallocatechin (EGC), and (−)-epigallocatechin-3-gallate (EGCG). Modulus of elasticity (E) and E fold increase were determined, comparing specimens at baseline and after treatment. Biodegradation rates were assessed by differences in percentage of dry mass before and after incubation with bacterial collagenase. The inhibition of MMP-9 and CT-B by 0.65%, 0.065% and 0.0065% of each catechin was determined using fluorimetric proteolytic assay kits. All monomeric catechins led to a significant increase in E. EGCG showed the highest E fold increase, followed by ECG, CG and GCG. EGCG, ECG, GCG and CG significantly lowered biodegradation rates and inhibited both MMP-9 and CT-B at a concentration of 0.65%. Overall, the 3-O-galloylated monomeric catechins are clearly more potent than their non-galloylated analogues in improving dentin mechanical properties, stabilizing collagen against proteolytic degradation, and inhibiting the activity of MMPs and CTs. The results indicate that galloylation is a key pharmacophore in the monomeric and likely also in the oligomeric PACs that exhibit high cross-linking potential for dentin extracellular matrix.
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DOI：
10.1016/j.jmbbm.2011.05.003
发表时间：
2011-10
影响因子：
3.9
作者：
Castellan, Carina Strano;Bedran-Russo, Ana Karina;Karol, Sachin;Rodrigues Pereira, Patricia Nobrega
通讯作者：
Rodrigues Pereira, Patricia Nobrega
影响因子：
6.1
作者：
Bras, Natercia F.;Goncalves, Rui;Do Freitas, Victor
通讯作者：
Do Freitas, Victor
影响因子：
6.1
作者：
Monagas, M;Gómez-Cordovés, C;Ricardo-Da-Silva, JM
通讯作者：
Ricardo-Da-Silva, JM
影响因子：
2.3
作者：
Sarni-Manchado, P;Cheynier, V
通讯作者：
Cheynier, V
影响因子：
7.6
作者：
Aguiar, T. R.;Vidal, C. M. P.;Bedran-Russo, A. K.
通讯作者：
Bedran-Russo, A. K.