Long-term stability of dentin matrix following treatment with various natural collagen cross-linkers.
Long-term stability of dentin matrix following treatment with various natural collagen cross-linkers.
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DOI：
10.1016/j.jmbbm.2011.05.003
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发表时间：
2011-10
影响因子：
3.9
通讯作者：
Rodrigues Pereira, Patricia Nobrega
中科院分区：
文献类型：
--
作者：
Castellan, Carina Strano;Bedran-Russo, Ana Karina;Karol, Sachin;Rodrigues Pereira, Patricia Nobrega
胶原蛋白解离是粘结剂修复失败的主要降解方式之一。这项研究的假设是，使用富含原花青素(PA)的天然胶原交联剂进行预处理，可以改善牙本质胶原的力学性能和稳定性，从而为粘接性修复提供更耐受和更持久的基材。将葡萄籽(GSE)、可可籽(CSE)、小红莓(CRE)、肉桂(CNE)和阿萨莓(ACE)的PA提取物涂在脱矿的牙本质上。对处理后的牙本质胶原蛋白的表观弹性模量(E)进行12个月的分析。将试件浸泡在各自的溶液中，在基线、10、30、60、120和240min进行微弯测试，获得E值。样本储存在人工唾液中，并在3个月、6个月和12个月后重新测试。数据分析采用方差分析和Tukey检验。GSE和CSE提取物表现出时间依赖效应，能够提高有机基质的E[240min(Mpa)：GSE=108.96±56.08；CSE=59.21±24.87]和稳定有机基质的E[12个月(Mpa)：GSE=40.91±19.69；CSE=42.11±13.46]。Cre和CNE提取物均能维持胶原基质的E恒定12个月以上[Cre=11.17±7.22；CNE=9.96±6.11；Mpa]。ACE2(2.64±1.22)Mpa和纯蒸馏水(1.37±0.69 Mpa)和乙醇水(0.95±0.33 Mpa)对牙本质有机基质无明显影响，但使其力学性能下降。部分PA类提取物通过外源交联剂的诱导，能够改善和稳定胶原基质。
Collagen disorganization is one of the main degradation patterns found in unsuccessful adhesive restorations. The hypothesis of this study was that pretreatment using natural collagen cross-linking agents rich in proanthocyanidin (PA) would improve mechanical properties and stability over time of the dentin collagen and, thus, confer a more resistant and lasting substrate for adhesive restorations. PA-based extracts, from grape seed (GSE), cocoa seed (CSE), cranberry (CRE), cinnamon (CNE) and açaí berry (ACE) were applied over the demineralized dentin. The apparent elastic modulus (E) of the treated dentin collagen was analyzed over a 12 months period. Specimens were immersed in the respective solution and E values were obtained by a micro-flexural test at baseline, 10, 30, 60, 120 and 240 min. Samples were stored in artificial saliva and re-tested after 3, 6 and 12 months. Data was analyzed using ANOVA and Tukey test. GSE and CSE extracts showed a time-dependant effect and were able to improve [240 min (MPa): GSE=108.96±56.08; CSE=59.21±24.87] and stabilize the E of the organic matrix [12 months (MPa): GSE=40.91±19.69; CSE= 42.11±13.46]. CRE and CNE extracts were able to maintain the E of collagen matrices constant over 12 months [CRE=11.17±7.22; CNE= 9,96±6.11; MPa]. ACE (2.64±1.22 MPa) and control groups immersed in neat distilled water (1.37±0.69 MPa) and ethanol water (0.95±0.33MPa) showed no effect over dentin organic matrix and enable their degradation and reduction of mechanical properties. Some PA-based extracts were capable of improving and stabilizing collagen matrices through exogenous cross-links induction.
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影响因子：
4.4
作者：
Castellan, Carina Strano;Pereira, Patricia N. R.;Viana, Grace;Chen, Shao-Nong;Pauli, Guido F.;Bedran-Russo, Ana Karina
通讯作者：
Bedran-Russo, Ana Karina
影响因子：
2.9
作者：
Matchett, MD;MacKinnon, SL;Hurta, RAR
通讯作者：
Hurta, RAR
影响因子：
1.9
作者：
Hara, AT;Queiroz, CS;Serra, MC
通讯作者：
Serra, MC
DOI：
10.1002/jmb.b.31022
发表时间：
2008-08-01
影响因子：
3.4
作者：
Bedran-Russo, Ana Karina B.;Pashley, David H.;Miescke, Klaus J.
通讯作者：
Miescke, Klaus J.
影响因子：
3
作者：
Martin-De las Heras, S;Valenzuela, A;Overall, CM
通讯作者：
Overall, CM