Analysis of the Pseudoalteromonas tunicata genome reveals properties of a surface-associated life style in the marine environment.
Analysis of the Pseudoalteromonas tunicata genome reveals properties of a surface-associated life style in the marine environment.
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DOI：
10.1371/journal.pone.0003252
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发表时间：
2008-09-24
期刊：
影响因子：
3.7
通讯作者：
Egan S
中科院分区：
文献类型：
--
作者：
Thomas T;Evans FF;Schleheck D;Mai-Prochnow A;Burke C;Penesyan A;Dalisay DS;Stelzer-Braid S;Saunders N;Johnson J;Ferriera S;Kjelleberg S;Egan S
细菌在固着真核生物宿主表面（例如无脊椎动物和海藻）的定植在海洋环境中是常见的，预计会产生显著的种间竞争和其他相互作用。被膜假交替单胞菌是海洋表面上成功的竞争者，主要是因为它能够产生一些抑制分子。因此，被衣假单胞菌已成为研究表面定殖和真核宿主-细菌相互作用过程的模式生物。为了更广泛地了解对表面相关生活方式的适应，我们对被衣青霉的基因组进行了测序和分析，并将其与密切相关菌株的基因组进行了比较。我们发现，被囊对虾基因组包含几个基因和基因簇，这些基因和基因簇参与产生抑制表面竞争者和次级定殖者的抑制化合物。被囊藻的氧化应激反应、铁清除和营养获取特征表明，该生物体对高密度表面群落具有良好的适应性。被囊紫菜基因组的变异由遗传重排和移动的遗传元件（例如转座子、CRISPR、噬菌体）的几个标志提出。表面附着可能由卷曲、新的皮利、许多细胞外聚合物和潜在的其他意想不到的细胞表面蛋白介导。被衣假单胞菌基因组还显示出细胞外聚合物的利用模式，这将避免其识别的宿主的降解，同时可能对其他宿主类型造成有害影响。此外，普遍存在的公认的毒力基因表明，披衣假单胞菌具有致病相互作用的潜力。基因组分析揭示了几个生理特征，将提供P. tunciata的竞争优势，对其他成员的表面相关的社区。我们还确定了可以介导与其目前公认的宿主以外的表面相互作用的特性。这与已知的毒力基因的检测一起导致了一种假设，即被衣假单胞菌在与一系列宿主表面的有益和有害相互作用之间保持着精心调节的平衡。
Colonisation of sessile eukaryotic host surfaces (e.g. invertebrates and seaweeds) by bacteria is common in the marine environment and is expected to create significant inter-species competition and other interactions. The bacterium Pseudoalteromonas tunicata is a successful competitor on marine surfaces owing primarily to its ability to produce a number of inhibitory molecules. As such P. tunicata has become a model organism for the studies into processes of surface colonisation and eukaryotic host-bacteria interactions. To gain a broader understanding into the adaptation to a surface-associated life-style, we have sequenced and analysed the genome of P. tunicata and compared it to the genomes of closely related strains. We found that the P. tunicata genome contains several genes and gene clusters that are involved in the production of inhibitory compounds against surface competitors and secondary colonisers. Features of P. tunicata's oxidative stress response, iron scavenging and nutrient acquisition show that the organism is well adapted to high-density communities on surfaces. Variation of the P. tunicata genome is suggested by several landmarks of genetic rearrangements and mobile genetic elements (e.g. transposons, CRISPRs, phage). Surface attachment is likely to be mediated by curli, novel pili, a number of extracellular polymers and potentially other unexpected cell surface proteins. The P. tunicata genome also shows a utilisation pattern of extracellular polymers that would avoid a degradation of its recognised hosts, while potentially causing detrimental effects on other host types. In addition, the prevalence of recognised virulence genes suggests that P. tunicata has the potential for pathogenic interactions. The genome analysis has revealed several physiological features that would provide P. tunciata with competitive advantage against other members of the surface-associated community. We have also identified properties that could mediate interactions with surfaces other than its currently recognised hosts. This together with the detection of known virulence genes leads to the hypothesis that P. tunicata maintains a carefully regulated balance between beneficial and detrimental interactions with a range of host surfaces.
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影响因子：
3.6
作者：
FONG, ST;CAMAKARIS, J;LEE, BTO
通讯作者：
LEE, BTO
影响因子：
3.7
作者：
Blanvillain S;Meyer D;Boulanger A;Lautier M;Guynet C;Denancé N;Vasse J;Lauber E;Arlat M
通讯作者：
Arlat M
影响因子：
3.2
作者：
Baynham, PJ;Ramsey, DM;Wozniak, DJ
通讯作者：
Wozniak, DJ
影响因子：
5.1
作者：
Bauer, Margarete;Kube, Michael;Gloeckner, Frank Oliver
通讯作者：
Gloeckner, Frank Oliver
DOI：
10.1099/ijs.0.64762-0
发表时间：
2007-06-01
影响因子：
2.8
作者：
Chiu, Hsiu-Hui;Shieh, Wung Yang;Wagner-Doebler, Irene
通讯作者：
Wagner-Doebler, Irene