Prey capture and phagocytosis in the choanoflagellate Salpingoeca rosetta.
Prey capture and phagocytosis in the choanoflagellate Salpingoeca rosetta.
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DOI：
10.1371/journal.pone.0095577
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
King N
中科院分区：
文献类型：
--
作者：
Dayel MJ;King N
领鞭毛虫是一种单细胞、群体性的水生微真核生物，其顶端鞭毛周围环绕着由肌动蛋白填充的微绒毛组成的摄食环，从而捕获细菌。顶端鞭毛产生的水流将被捕食的细菌驱赶到食环进行吞噬。我们在这里报告的细胞生物学捕获猎物的玫瑰花状菌落和单细胞的“囊”或基板附着细胞从choanoflagellate S。罗塞塔。在泡囊细胞和玫瑰花结菌落中，吞噬作用最初涉及多个微绒毛的融合，随后重塑领膜以吞噬猎物，并将被吞噬的细菌运输到细胞中。尽管泡囊细胞和玫瑰花结集落细胞都能产生约70 nm的“领链”，连接并潜在地稳定相邻的微绒毛，但仅观察到泡囊细胞产生一个片状的“领裙”，环绕着领的基部。本研究为深入了解中华绒螯蟹摄食猎物的过程提供了依据。Rosetta的，并提供了一个背景下，考虑在choanoflagellates的孤细胞和殖民地之间的潜在生态差异。
Choanoflagellates are unicellular and colonial aquatic microeukaryotes that capture bacteria using an apical flagellum surrounded by a feeding collar composed of actin-filled microvilli. Flow produced by the apical flagellum drives prey bacteria to the feeding collar for phagocytosis. We report here on the cell biology of prey capture in rosette-shaped colonies and unicellular “thecate” or substrate attached cells from the choanoflagellate S. rosetta. In thecate cells and rosette colonies, phagocytosis initially involves fusion of multiple microvilli, followed by remodeling of the collar membrane to engulf the prey, and transport of engulfed bacteria into the cell. Although both thecate cells and rosette colony cells produce ∼70 nm “collar links” that connect and potentially stabilize adjacent microvilli, only thecate cells were observed to produce a lamellipod-like “collar skirt” that encircles the base of the collar. This study offers insight into the process of prey ingestion by S. rosetta, and provides a context within which to consider potential ecological differences between solitary cells and colonies in choanoflagellates.
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DOI：
10.1073/pnas.0801667105
发表时间：
2008-10-28
影响因子：
11.1
作者：
Carr, M.;Leadbeater, B. S. C.;Baldauf, S. L.
通讯作者：
Baldauf, S. L.
影响因子：
2.2
作者：
Karpov, SA;Leadbeater, BSC
通讯作者：
Leadbeater, BSC
影响因子：
1.6
作者：
Leys, Sally P.;Eerkes-Medrano, Dafne I.
通讯作者：
Eerkes-Medrano, Dafne I.
影响因子：
1.2
作者：
Eerkes-Medrano, Dafne I.;Leys, Sally P.
通讯作者：
Leys, Sally P.
影响因子：
2.7
作者：
Dayel, Mark J.;Alegado, Rosanna A.;King, Nicole
通讯作者：
King, Nicole