Proteomic analysis of spermathecal fluid reveals factors related to long-term sperm storage in ant queens
Proteomic analysis of spermathecal fluid reveals factors related to long-term sperm storage in ant queens
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受精囊液的蛋白质组学分析揭示了与蚁后精子长期储存相关的因素
DOI：
10.1101/2022.11.02.513948
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Gotoh Ayako
中科院分区：
文献类型：
--
作者：
江成広斗;髙田真大朗;Gotoh Ayako
蚁后在成年期开始交配后，可以在十多年内维持大量的精子细胞，直到死亡。这种能力很突出，因为动物射精后精子细胞无法长期保持受精能力;然而，细胞机制仍不清楚。精子细胞保存在女性精子储存器官受精囊中，受精囊为精子细胞提供了合适的环境。为了揭示蚁后精子长期储存机制的分子基础，采用基于质谱的蛋白质组学技术，对日本蚁受精囊液中相对于血淋巴富集的蛋白质进行了鉴定。与细胞外基质、抗氧化剂、代谢途径、蛋白酶相关的蛋白质和具有未知功能的蛋白质相对于血淋巴在受精囊液中富集。这些富集的蛋白质与先前通过对Crematogaster osakensis蜂王受精囊的转录组分析检测到的高表达基因是共享的，而Crematogaster osakensis属于不同于L. - 是的人们认为，长期储存精子的能力是在早期蚂蚁谱系中进化出来的;因此，在两种蚂蚁中鉴定出的共同蛋白质对这种能力至关重要。
Ant queens can maintain a large number of sperm cells for over a decade after mating at the beginning of their adult lives until they die. This ability is prominent because sperm cells cannot maintain their fertilization ability long after ejaculation in animals; however, the cellular mechanisms remain unclear. Sperm cells are maintained in the female sperm storage organ, the spermatheca, which supplies a suitable environment for sperm cells. To reveal the molecular basis of the long-term sperm storage mechanisms in ant queens, protein profiles enriched in the spermathecal fluid relative to the hemolymph were identified inLasius japonicususing mass spectrometry-based proteomics. Proteins related to the extracellular matrix, antioxidant, metabolic pathways, proteases, and with uncharacterized functions were enriched in the spermathecal fluid relative to the hemolymph. These enriched proteins were shared with highly expressed genes previously detected by transcriptome analyses of the spermatheca in queens ofCrematogaster osakensisbelonging to a different subfamily thanL. japonicus. It is considered that the ability for long-term sperm storage has evolved in the early ant lineage; therefore, the common proteins identified in the two ant species are crucial for this ability.
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影响因子：
4.4
作者：
Kawashima, Yusuke;Nagai, Hirotaka;Konno, Ryo;Ishikawa, Masaki;Nakajima, Daisuke;Sato, Hironori;Nakamura, Ren;Furuyashiki, Tomoyuki;Ohara, Osamu
通讯作者：
Ohara, Osamu
近缺氧导致蚁后精子固定并维持活力：对延长精子储存机制的新见解
DOI：
10.1101/2022.09.08.507031
发表时间：
2022
期刊：
bioRxiv
影响因子：
--
作者：
A. Gotoh;Mika Takeshima;Ken
通讯作者：
Ken
深入了解切叶蚁 Atta sexdens rubropilosa（膜翅目：蚁科）受精囊蛋白质组
影响因子：
2.5
作者：
Juliana Malta;G. Martins;A. E. Marques;P. Games;J. Zanuncio;M. C. Baracat;T. Salomão
通讯作者：
T. Salomão
DOI：
10.1073/pnas.2119899119
发表时间：
2022-03-15
影响因子：
11.1
作者：
McCullough EL;Whittington E;Singh A;Pitnick S;Wolfner MF;Dorus S
通讯作者：
Dorus S
影响因子：
14.9
作者：
UniProt Consortium
通讯作者：
UniProt Consortium