De Novo Sequencing and Transcriptome Analysis of Pleurotus eryngii subsp. tuoliensis (Bailinggu) Mycelia in Response to Cold Stimulation.
De Novo Sequencing and Transcriptome Analysis of Pleurotus eryngii subsp. tuoliensis (Bailinggu) Mycelia in Response to Cold Stimulation.
复制标题
杏鲍菇托里菌亚种菌丝体对冷刺激反应的从头测序和转录组分析
DOI：
10.3390/molecules21050560
复制
发表时间：
2016-05-17
期刊：
影响因子：
--
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Fu YP;Liang Y;Dai YT;Yang CT;Duan MZ;Zhang Z;Hu SN;Zhang ZW;Li Y
白铃菇菌丝体的冷刺激是在商业栽培条件下引发原基起始、成功产生子实体的主要因素。然而，菌丝对冷刺激反应的分子水平机制尚不清楚。本研究利用RNA-Seq技术进行转录组比较分析，以更好地了解白铃谷在不同时间刺激阶段的基因表达调控。从4个文库（对照温度为25℃，外加冷刺激温度为- 3℃，处理时间分别为1-2天、5-6天和9-10天）中重新组装和注释了共21558个白灵谷单一菌丝体。GO和KEGG通路分析表明，与细胞壁和细胞膜稳定、钙信号和丝裂原活化蛋白激酶（MAPK）通路以及可溶性糖和蛋白的生物合成和代谢通路相关的差异表达的功能群在白铃谷对冷刺激的反应中起重要作用。在这些单基因中鉴定出了667个潜在的EST-SSR位点，并随机选取了100个EST-SSR引物进行验证。10个白灵谷野生菌株的总体多态性率为92%。因此，这些结果可以为更好地了解白铃谷对冷刺激反应的分子机制提供有价值的资源。
Cold stimulation of Bailinggu’s mycelia is the main factor that triggers primordia initiation for successful production of fruiting bodies under commercial cultivation. Yet, the molecular-level mechanisms involved in mycelia response to cold stimulation are still unclear. Here, we performed comparative transcriptomic analysis using RNA-Seq technology to better understand the gene expression regulation during different temporal stages of cold stimulation in Bailinggu. A total of 21,558 Bailinggu mycelia unigenes were de novo assembled and annotated from four libraries (control at 25 °C, plus cold stimulation treatments at −3 °C for a duration of 1–2 days, 5–6 days, and 9–10 days). GO and KEGG pathway analysis indicated that functional groups of differentially expressed unigenes associated with cell wall and membrane stabilization, calcium signaling and mitogen-activated protein kinases (MAPK) pathways, and soluble sugars and protein biosynthesis and metabolism pathways play a vital role in Bailinggu’s response to cold stimulation. Six hundred and seven potential EST-based SSRs loci were identified in these unigenes, and 100 EST-SSR primers were randomly selected for validation. The overall polymorphism rate was 92% by using 10 wild strains of Bailinggu. Therefore, these results can serve as a valuable resource for a better understanding of the molecular mechanisms associated with Bailinggu’s response to cold stimulation.
登录
查看更多内容
拟南芥冷冻和冷冻后恢复过程中质体外和质体脂质的差异降解
影响因子：
4.8
作者：
Li, Weiqi;Wang, Ruiping;Wang, Xuemin
通讯作者：
Wang, Xuemin
影响因子：
3.7
作者：
Chen X;Zhang J;Liu Q;Guo W;Zhao T;Ma Q;Wang G
通讯作者：
Wang G
影响因子：
5.8
作者：
Leng, Ning;Dawson, John A.;Kendziorski, Christina
通讯作者：
Kendziorski, Christina
影响因子：
5.4
作者：
Han, ZG;Wang, CB;Zhang, TZ
通讯作者：
Zhang, TZ
影响因子：
6.7
作者：
Bayry J;Aimanianda V;Guijarro JI;Sunde M;Latgé JP
通讯作者：
Latgé JP