Transcriptome sequencing and identification of cold tolerance genes in hardy Corylus species (C. heterophylla Fisch) floral buds.
Transcriptome sequencing and identification of cold tolerance genes in hardy Corylus species (C. heterophylla Fisch) floral buds.
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DOI：
10.1371/journal.pone.0108604
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Chen X;Zhang J;Liu Q;Guo W;Zhao T;Ma Q;Wang G
榛属植物是我国东北地区重要的木本植物。其产品榛子是糕点和巧克力行业最重要的原材料之一。然而，有限的遗传研究集中在榛属，因为缺乏基因组资源。高通量测序技术的出现为榛属植物的研究提供了一个转折点。在本研究中，我们进行了从头转录组测序的第一次，以产生一个全面的数据库，榛子菲施花芽。梭采用Illumina双末端测序技术对异叶菲施花芽转录组进行测序。我们产生了28，930，890个原始读段，并将它们组装成82，684个重叠群。共鉴定了40，941个单基因，其中30，549个在NCBI非冗余（Nr）蛋白质数据库中注释，18，581个在Swiss-Prot数据库中注释。在这些注释的unigenes中，分别有25，311和10，514个unigenes被分配到基因本体（GO）类别和直向同源群（COG）簇。我们可以使用京都基因和基因组通路百科全书（KEGG）数据库将17，207个单基因映射到128个通路上。此外，基于转录组，我们构建了一个C.异叶菲施花蕾。筛选出的基因在冷适应的4个阶段的表达模式表明，这些基因可能参与了冷适应的不同阶段。异叶菲施花蕾。C. heterophylla菲施花芽的基因组序列进行了深度测序、从头组装和注释，为更好地理解C.异叶菲施花芽转录组。初步鉴定了与冷胁迫相关的候选基因，为进一步研究冷胁迫的分子机制奠定了物质基础。异叶菲施花芽耐冷胁迫。
The genus Corylus is an important woody species in Northeast China. Its products, hazelnuts, constitute one of the most important raw materials for the pastry and chocolate industry. However, limited genetic research has focused on Corylus because of the lack of genomic resources. The advent of high-throughput sequencing technologies provides a turning point for Corylus research. In the present study, we performed de novo transcriptome sequencing for the first time to produce a comprehensive database for the Corylus heterophylla Fisch floral buds. The C. heterophylla Fisch floral buds transcriptome was sequenced using the Illumina paired-end sequencing technology. We produced 28,930,890 raw reads and assembled them into 82,684 contigs. A total of 40,941 unigenes were identified, among which 30,549 were annotated in the NCBI Non-redundant (Nr) protein database and 18,581 were annotated in the Swiss-Prot database. Of these annotated unigenes, 25,311 and 10,514 unigenes were assigned to gene ontology (GO) categories and clusters of orthologous groups (COG), respectively. We could map 17,207 unigenes onto 128 pathways using the Kyoto Encyclopedia of Genes and Genomes Pathway (KEGG) database. Additionally, based on the transcriptome, we constructed a candidate cold tolerance gene set of C. heterophylla Fisch floral buds. The expression patterns of selected genes during four stages of cold acclimation suggested that these genes might be involved in different cold responsive stages in C. heterophylla Fisch floral buds. The transcriptome of C. heterophylla Fisch floral buds was deep sequenced, de novo assembled, and annotated, providing abundant data to better understand the C. heterophylla Fisch floral buds transcriptome. Candidate genes potentially involved in cold tolerance were identified, providing a material basis for future molecular mechanism analysis of C. heterophylla Fisch floral buds tolerant to cold stress.
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影响因子：
2
作者：
Faccioli, P;Pecchioni, N;Terzi, V
通讯作者：
Terzi, V
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DOI：
10.1083/jcb.136.6.1307
发表时间：
1997-03-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
Carlier MF;Laurent V;Santolini J;Melki R;Didry D;Xia GX;Hong Y;Chua NH;Pantaloni D
通讯作者：
Pantaloni D
影响因子：
3.3
作者：
Fan, Lanfen;Wang, Anli;Wu, Yingxia
通讯作者：
Wu, Yingxia
影响因子：
7.2
作者：
Cabello, Julieta V.;Arce, Agustin L.;Chan, Raquel L.
通讯作者：
Chan, Raquel L.
影响因子：
1
作者：
Erdogan, V;Mehlenbacher, SA
通讯作者：
Mehlenbacher, SA