Transcriptome Analysis of the Regulatory Mechanism of FoxO on Wing Dimorphism in the Brown Planthopper, Nilaparvata lugens (Hemiptera: Delphacidae).
Transcriptome Analysis of the Regulatory Mechanism of FoxO on Wing Dimorphism in the Brown Planthopper, Nilaparvata lugens (Hemiptera: Delphacidae).
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FoxO对褐飞虱翅二态性调控机制的转录组分析（半翅目：飞虱科）
DOI：
10.3390/insects12050413
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发表时间：
2021-05-04
期刊：
影响因子：
3
通讯作者：
Xu HJ
中科院分区：
文献类型：
--
作者：
Xu N;Wei SF;Xu HJ
褐飞虱（Nilaparvata lugens）在幼虫期可发育成长翅或短翅型成虫，这取决于所接受的环境刺激。转录因子NlFoxO作为一个关键的调节器，确定在BPH的替代翼形态，但基本的分子机制是未知的。在这里，我们调查了转录组档案的前翅和后翅芽整个5龄阶段，翅形态决定阶段。结果表明，NlFoxO主要通过调控细胞增殖相关基因的表达来调控翅芽的发育可塑性。摘要褐飞虱（Nilaparvata lugens）成虫可发育成短翅型或长翅型，并长期作为研究昆虫翅型多型化机制的模式生物。转录因子NlFoxO作为指导SW或LW变体发育的主调节因子，但其潜在的分子机制在很大程度上是未知的。在这里，我们用靶向NlFoxO（dsNlFoxO）的双链RNA（dsRNA）显微注射SW-注定的变体以将它们改变成LW-有翼变体。平行地，用靶向编码绿色荧光蛋白（dsGfp）的基因的dsRNA显微注射SW-目的变体作为阴性对照。在羽化后24、36和48 h采集5龄蝗虫的前翅和后翅芽（hAE）用于RNA测序。我们在单个发育时间从前翅和后翅芽获得了至少4340万个干净的读数。差异表达基因（DEG）在各种基因本体（GO）术语中显著富集，包括细胞过程、结合和细胞部分。京都基因和基因组百科全书（KEGG）富集途径分析表明，在dsNlFoxO处理的前翅和后翅芽中上调的基因在很大程度上与细胞周期和DNA复制相关。此外，大多数上调的基因显示更高的表达在24-，和36-hAE相对于48 hAE，表明翼细胞在LW-注定的翅膀可能积极增殖在第一个36小时在5龄蛹。我们的研究结果表明，LW在BPH的发展可能是依赖于细胞增殖的持续时间在5龄阶段，这揭示了昆虫翅多态性的分子基础。
Simple Summary The brown planthopper (BPH) Nilaparvata lugens can develop into either long-winged or short-winged adults depending on environmental stimuli received during larval stages. The transcription factor NlFoxO serves as a key regulator determining alternative wing morphs in BPH, but the underlying molecular mechanism is largely unknown. Here, we investigated the transcriptomic profile of forewing and hindwing buds across the 5th-instar stage, the wing-morph decision stage. Our results indicated that NlFoxO modulated the developmental plasticity of wing buds mainly by regulating the expression of cell proliferation-associated genes. Abstract The brown planthopper (BPH), Nilaparvata lugens, can develop into either short-winged (SW) or long-winged (LW) adults according to environmental conditions, and has long served as a model organism for exploring the mechanisms of wing polyphenism in insects. The transcription factor NlFoxO acts as a master regulator that directs the development of either SW or LW morphs, but the underlying molecular mechanism is largely unknown. Here, we microinjected SW-destined morphs with double stranded-RNA (dsRNA) targeting NlFoxO (dsNlFoxO) to change them into LW-winged morphs. In parallel, SW-destined morphs microinjected with dsRNA targeting the gene encoding green fluorescence protein (dsGfp) served as a negative control. The forewing and hindwing buds of 5th-instar nymphs collected at 24, 36, and 48 h after eclosion (hAE) were used for RNA sequencing. We obtained a minimum of 43.4 million clean reads from forewing and hindwing buds at a single developmental time. Differentially expressed genes (DEGs) were significantly enriched in various Gene Ontology (GO) terms, including cellular process, binding, and cell part. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment pathway analysis showed that up-regulated genes in dsNlFoxO-treated forewing and hindwing buds were largely associated with the cell cycle and DNA replication. Furthermore, most up-regulated genes displayed higher expression at 24-, and 36-hAE relative to 48 hAE, indicating that wing cells in LW-destined wings might actively proliferate during the first 36 h in 5th-instar nymphs. Our findings indicated that LW development in BPH was likely dependent on the duration of cell proliferation in the 5th-instar stage, which sheds light on the molecular basis of wing polymorphism in insects.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
5.2
作者：
Otuka A
通讯作者：
Otuka A
DOI：
10.1895/wormbook.1.164.1
发表时间：
2013-12-26
期刊：
WormBook : the online review of C. elegans biology
影响因子：
--
作者：
Murphy, Coleen T;Hu, Patrick J
通讯作者：
Hu, Patrick J
影响因子：
64.8
作者：
Medema, RH;Kops, GJPL;Burgering, BMT
通讯作者：
Burgering, BMT
影响因子：
--
作者：
Kramer, Jamie M;Davidge, Jason T;Staveley, Brian E
通讯作者：
Staveley, Brian E