Effects of social isolation on 50-kHz ultrasonic vocalizations, affective state, cognition, and neurotransmitter concentrations in the ventral tegmental and locus coeruleus of adult rats.
Effects of social isolation on 50-kHz ultrasonic vocalizations, affective state, cognition, and neurotransmitter concentrations in the ventral tegmental and locus coeruleus of adult rats.
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DOI：
10.1016/j.bbr.2022.114157
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发表时间：
2023-02-02
影响因子：
2.7
通讯作者：
Ciucci, Michelle R.
中科院分区：
文献类型：
--
作者：
Broadfoot, Courtney K.;Lenell, Charles;Kelm-Nelson, Cynthia A.;Ciucci, Michelle R.
声音交流、认知和情感状态是持续健康的关键特征，并且由于发声通常是出于社会动机，因此社会经验可能在这些行为中发挥作用。腹侧被盖区 (VTA) 和蓝斑 (LC) 的单胺能系统与社会和奖励处理、发声产生以及响应环境压力的神经递质变化有关。社会隔离对这些复杂行为和潜在神经机制的影响相对未知。为了补充这一文献，我们将成年雄性 Long-Evans 大鼠随机分为对照（与笼友一起饲养）或隔离（单独饲养）条件，并在 2、4、6、8 和 10 个月龄时测定超声波发声、认知（新物体识别测试）、焦虑（高架十字迷宫）和快感缺失（蔗糖偏好测试）。 10 个月时，使用高效液相色谱法测定 VTA 和 LC 样本中的多巴胺、去甲肾上腺素和血清素。我们测试了以下假设：隔离 1) 会减少发声和认知，2) 会增加焦虑和抑郁，3) 会增加 VTA 和 LC 中的多巴胺、去甲肾上腺素和血清素水平。结果显示，隔离显着降低了发声音调（信噪比）并增加了最大频率。认知、焦虑或快感缺乏方面没有显着的发现。离体大鼠 VTA 中的多巴胺和血清素及其各自的代谢物显着增加。这些发现表明，社会条件的慢性变化（例如隔离）会影响发声的产生和 VTA 神经递质的水平。
Vocal communication, cognition, and affective state are key features of sustained health and wellness, and because vocalizations are often socially-motivated, social experience likely plays a role in these behaviors. The monoaminergic systems of the ventral tegmental area (VTA) and the locus coeruleus (LC) are associated with social and reward processing, vocalization production, and neurotransmitter changes in response to environmental stressors. The effect of social isolation on these complex behaviors and the underlying neural mechanisms is relatively unknown. To add to this body of literature, we randomized adult male Long-Evans rats to control (housed with a cagemate) or isolated (housed individually) conditions and assayed ultrasonic vocalizations, cognition (novel object recognition test), anxiety (elevated plus maze) and anhedonia (sucrose preference test) at 2, 4, 6, 8, and 10 months of age. At 10 months, VTA and LC samples were assayed for dopamine, norepinephrine, and serotonin using high performance liquid chromatography. We tested the hypotheses that isolation 1) diminishes vocalizations and cognition, 2) increases anxiety and depression, and 3) increases levels of dopamine, norepinephrine, and serotonin in the VTA and LC. Results showed isolation significantly reduced vocalization tonality (signal-to-noise ratio) and increased maximum frequency. There were no significant findings for cognition, anxiety, or anhedonia. Dopamine and serotonin and their respective metabolites were significantly increased in the VTA in isolated rats. These findings suggest chronic changes to social condition such as isolation affects vocalization production and levels of VTA neurotransmitters.
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影响因子：
2.6
作者：
Brudzynski, SM
通讯作者：
Brudzynski, SM
影响因子：
2.7
作者：
Alshammari, Tahani K.;Alghamdi, Hajar;Alshammari, Musaad A.
通讯作者：
Alshammari, Musaad A.
影响因子：
2
作者：
Basken, Jaime N.;Connor, Nadine P.;Ciucci, Michelle R.
通讯作者：
Ciucci, Michelle R.
影响因子：
2.7
作者：
Borges, Juliano Viana;de Freitas, Betania Souza;Bromberg, Elke
通讯作者：
Bromberg, Elke
影响因子：
1.7
作者：
Ciucci, Michelle R.;Vinney, Lisa;Wahoske, Emerald J.;Connor, Nadine P.
通讯作者：
Connor, Nadine P.