Hypobaric hypoxia induced renal damage is mediated by altering redox pathway.
Hypobaric hypoxia induced renal damage is mediated by altering redox pathway.
复制标题
DOI：
10.1371/journal.pone.0195701
复制
发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Reddy MPK
中科院分区：
文献类型：
--
作者：
Chhabra V;Anand AS;Baidya AK;Malik SM;Kohli E;Reddy MPK
据报道，全身性低压缺氧可导致肾脏损害，但确切的病理生理机制尚不完全清楚。因此，本研究旨在用蛋白质组学方法探讨低压低氧条件下肾脏的病理生理机制。将6~8周龄雄性SD大鼠暴露于相当于海拔7628米(pO2-282 mm hg)、温度28℃、湿度55%的减压舱内，持续1、3、7天。采用生理学、蛋白质组学和生物信息学方法研究慢性低压缺氧对肾脏的影响。我们的数据显示，随着低压低氧暴露时间的延长，轻度至中度退行性肾小管改变，肾功能改变，损伤生物标志物和收缩压。肾脏蛋白质组学分析显示，与正常氧对照组相比，低氧暴露7d组大鼠肾脏蛋白质组有38个差异表达点，其中25个点表达下调，13个点表达上调。鉴定出的蛋白质参与了与内源性氧化还原途径、细胞完整性和能量代谢相关的特定分子变化。这项研究通过研究生理和蛋白质组学的变化，为低压缺氧下肾脏的动态平衡提供了经验证据。明确的关键蛋白的鉴定为研究低压缺氧下氧化还原信号介导的肾脏损伤提供了有价值的线索。
Systemic hypobaric hypoxia is reported to cause renal damage; nevertheless the exact pathophysiological mechanisms are not completely understood. Therefore, the present study aims to explore renal pathophysiology by using proteomics approach under hypobaric hypoxia. Six to eight week old male Sprague Dawley rats were exposed to hypobaric hypoxia equivalent to altitude of 7628 metres (pO2-282mmhg) at 28°C and 55% humidity in decompression chamber for different time intervals; 1, 3, and7 days. Various physiological, proteomic and bioinformatic studies were carried out to examine the effect of chronic hypobaric hypoxia on kidney. Our data demonstrated mild to moderate degenerative tubular changes, altered renal function, injury biomarkers and systolic blood pressure with increase in duration of hypobaric hypoxia exposure. Renal proteomic analysis showed 38 differential expressed spots, out of which 25 spots were down regulated and 13 were up regulated in 7 dayhypobarichypoxic exposure group of rats as compared to normoxia control. Identified proteins were involved in specific molecular changes pertinent to endogenous redox pathways, cellular integrity and energy metabolism. The study provides an empirical evidence of renal homeostasis under hypobaric hypoxia by investigating both physiological and proteomics changes. The identification of explicit key proteins provides a valuable clue about redox signalling mediated renal damage under hypobaric hypoxia.
登录
查看更多内容
大鼠脑海马中的性低氧缺氧失衡线粒体动力学。
影响因子：
1.5
作者：
Jain K;Prasad D;Singh SB;Kohli E
通讯作者：
Kohli E
影响因子：
3.8
作者：
Ilic Z;Crawford D;Vakharia D;Egner PA;Sell S
通讯作者：
Sell S
影响因子：
4
作者：
Debevec T;Millet GP;Pialoux V
通讯作者：
Pialoux V
高海拔生理学和病理生理学：对重症监护医学的影响和相关性。
DOI：
10.1186/cc5142
发表时间：
2007
期刊：
Critical care (London, England)
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.4
作者：
Hurtado-Arestegui, Abdias;Plata-Cornejo, Raul;Pando, Jackelina
通讯作者：
Pando, Jackelina