A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems.

A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems.
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炔标签拉曼成像(ATRI)的十年:在生物系统中的应用。

DOI:
10.1039/d1cb00116g
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发表时间:
2021-10-07
影响因子:
4.1
通讯作者:
Sodeoka M
Sodeoka M
中科院分区:
其他
文献类型:
--
作者:
Bakthavatsalam S;Dodo K;Sodeoka M

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炔官能团在不受细胞成分干扰的区域(1800 cm−1至2800 cm−1)具有拉曼特征,该区域称为“沉默区域”,十年前首次利用该区域的炔信号来可视化胸腺嘧啶核苷类似物EDU的核定位。从那时起,利用炔基官能团对生物样品进行拉曼成像的策略,被称为炔基标记拉曼成像(ATRI),得到了广泛的应用。本文综述了ATRI在从细胞器到全动物模型的生物样品中的应用,并简要讨论了该技术的前景。炔类官能团在细胞无声区具有独特的伸缩频率。本文综述了乙炔标记在生物样品拉曼成像中的应用。
Alkyne functional groups have Raman signatures in a region (1800 cm−1 to 2800 cm−1) that is free from interference from cell components, known as the “silent region”, and alkyne signals in this region were first utilized a decade ago to visualize the nuclear localization of a thymidine analogue EdU. Since then, the strategy of Raman imaging of biological samples by using alkyne functional groups, called alkyne-tag Raman imaging (ATRI), has become widely used. This article reviews the applications of ATRI in biological samples ranging from organelles to whole animal models, and briefly discusses the prospects for this technique. Alkyne functional groups have unique stretching frequency in the cell silent region. This review discusses the application of alkyne tags for Raman imaging in biological samples.
使用基于双芳基丁二炔的探针通过高光谱受激拉曼散射显微镜对活细胞进行耐光溶酶体成像
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