Adult-specific electrical silencing of pacemaker neurons uncouples molecular clock from circadian outputs.
Adult-specific electrical silencing of pacemaker neurons uncouples molecular clock from circadian outputs.
复制标题
DOI：
10.1016/j.cub.2011.09.027
复制
发表时间：
2011-11-08
期刊：
影响因子：
--
通讯作者：
Ceriani MF
中科院分区：
文献类型：
--
作者：
Depetris-Chauvin A;Berni J;Aranovich EJ;Muraro NI;Beckwith EJ;Ceriani MF
昼夜节律通过在特定起搏器细胞内运行的时钟基因的转录反馈环来调节生理和行为。在果蝇中，小腹侧神经元(SLNvS)的分子振荡控制着自由奔跑条件下的节律性行为，以昼夜节律的方式释放神经肽色素分散因子(PDF)。SLNvS中的电活动也是行为节律性所必需的。然而，时间信息如何转化为行为仍不清楚。在这里，我们开发了一种新的工具，用于时间控制基因表达，以获得成人限制性的PDF电路的电沉默，这导致可逆性行为心律失常。值得注意的是，静默阶段的PER振荡没有改变，这表明心律失常是静默活动的直接结果。因此，昼夜节律轴突重构和PDF积聚在静默期受到严重影响。虽然sLNvS的电活动不是时钟组件，但它协调导致节律行为的电路输出。
Circadian rhythms regulate physiology and behavior through transcriptional feedback loops of clock genes running within specific pacemaker cells. In Drosophila, molecular oscillations in the small ventral Lateral Neurons (sLNvs) command rhythmic behavior under free-running conditions releasing the neuropeptide PIGMENT DISPERSING FACTOR (PDF) in a circadian fashion. Electrical activity in the sLNvs is also required for behavioral rhythmicity. Yet, how temporal information is transduced into behavior remains unclear. Here we developed a new tool for temporal control of gene expression to obtain adult-restricted electrical silencing of the PDF circuit, which led to reversible behavioral arrhythmicity. Remarkably, PER oscillations during the silenced phase remained unaltered, indicating that arrhythmicity is a direct consequence of the silenced activity. Accordingly, circadian axonal remodeling and PDF accumulation were severely affected during the silenced phase. Although electrical activity of the sLNvs is not a clock component it coordinates circuit outputs leading to rhythmic behavior.
登录
查看更多内容
影响因子：
9.8
作者：
Lear BC;Zhang L;Allada R
通讯作者：
Allada R
影响因子：
64.5
作者：
Nitabach, MN;Blau, J;Holmes, TC
通讯作者：
Holmes, TC
影响因子：
56.9
作者：
MICHEL, S;GEUSZ, ME;BLOCK, GD
通讯作者：
BLOCK, GD
影响因子：
5.3
作者：
Harrisingh, Marie C.;Wu, Ying;Nitabach, Michael N.
通讯作者：
Nitabach, Michael N.
影响因子：
64.8
作者：
Grima, B;Chèlot, E;Rouyer, F
通讯作者：
Rouyer, F