Lighting up left-handed Z-DNA: photoluminescent carbon dots induce DNA B to Z transition and perform DNA logic operations.
Lighting up left-handed Z-DNA: photoluminescent carbon dots induce DNA B to Z transition and perform DNA logic operations.
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点亮左手Z-DNA：光致发光碳点诱导DNA B到Z跃迁并执行DNA逻辑运算
DOI：
10.1093/nar/gkt575
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发表时间：
2013-09
影响因子：
14.9
通讯作者：
Qu X
中科院分区：
文献类型：
--
作者：
Feng L;Zhao A;Ren J;Qu X
左手Z-DNA是一种发生在转录过程中的瞬时结构。DNA B-Z转换不仅具有重要的生物学意义，而且与疾病和DNA纳米技术的关系也引起了人们的极大关注。近年来，光致发光碳点，特别是高发光氮掺杂碳点，因其低毒、高稳定性、表面功能可控等优点，在生物成像和基因/药物传递等方面的应用引起了人们的极大关注。然而，目前还不清楚碳点是否会影响DNA的构象或结构转变，如B-Z转变。在此，基于我们之前关于DNA与碳纳米管相互作用的系列工作，我们报道了第一个例子，即在生理盐条件下，光致发光碳点可以诱导右手B-DNA变成左手Z-DNA，并具有序列和构象选择性。进一步的研究表明，碳点将以GC优先的方式结合到DNA主槽上。受碳点照亮Z-DNA和DNA纳米技术的启发，基于荧光碳点和DNA嵌入器之间的荧光共振能量转移，设计并构建了几种类型的DNA逻辑门。
Left-handed Z-DNA has been identified as a transient structure occurred during transcription. DNA B-Z transition has attracted much attention because of not only Z-DNA biological importance but also their relation to disease and DNA nanotechnology. Recently, photoluminescent carbon dots, especially highly luminescent nitrogen-doped carbon dots, have attracted much attention on their applications to bioimaging and gene/drug delivery because of carbon dots with low toxicity, highly stable photoluminescence and controllable surface function. However, it is still unknown whether carbon dots can influence DNA conformation or structural transition, such as B-Z transition. Herein, based on our previous series work on DNA interactions with carbon nanotubes, we report the first example that photoluminescent carbon dots can induce right-handed B-DNA to left-handed Z-DNA under physiological salt conditions with sequence and conformation selectivity. Further studies indicate that carbon dots would bind to DNA major groove with GC preference. Inspired by carbon dots lighting up Z-DNA and DNA nanotechnology, several types of DNA logic gates have been designed and constructed based on fluorescence resonance energy transfer between photoluminescent carbon dots and DNA intercalators.
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