Water absorption through salivary gland type I acini in the blacklegged tick, Ixodes scapularis.
Water absorption through salivary gland type I acini in the blacklegged tick, Ixodes scapularis.
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作者：
Kim D;Maldonado-Ruiz P;Zurek L;Park Y
蜱的唾液腺在维持水平衡以维持生存方面起着关键作用，因为它们在宿主吸血期间消除多余的水和离子。在无宿主期间的长时间禁食中，蜱虫会将吸湿唾液分泌到口腔中，以吸收大气中的水蒸气。根据超微结构研究，推测蜱类唾液腺的I型腺泡参与了吸湿唾液的分泌。然而，我们最近提出I型腺泡在从由其他唾液腺泡产生的初级唾液中再吸收水/离子中起作用（即，II型和III型）。在这项研究中，我们测试的功能，I型腺泡在未喂养的女性肩突硬蜱。在用荧光染料罗丹明123强制喂食水后，跟踪摄入水的途径。我们发现，I型腺泡的唾液腺，而不是II型和III型，负责水的吸收。此外，还观察到通过中肠摄入水。哇巴因，一种Na/K-ATP酶抑制剂，注射或喂养，抑制水的吸收在I型腺泡。当我。当给肩胛骨蜱一滴水时，蜱很少直接吸水（5%），而有些蜱则接近水滴以利用水滴附近形成的高湿度（23%）。我们的结论是，在这两个主机和关闭阶段，I型腺泡在唾液腺的雌性肩突硬蜱吸收水和离子。
Tick salivary glands play critical roles in maintaining water balance for survival, as they eliminate excess water and ions during blood feeding on hosts. In the long duration of fasting in the off-host period, ticks secrete hygroscopic saliva into the mouth cavity to uptake atmospheric water vapor. Type I acini of tick salivary glands are speculated to be involved in secretion of hygroscopic saliva based on ultrastructure studies. However, we recently proposed that type I acini play a role in resorption of water/ions from the primary saliva produced by other salivary acini (i.e., types II and III) during the tick blood feeding phase. In this study, we tested the function of type I acini in unfed female Ixodes scapularis. The route of ingested water was tracked after forced feeding of water with fluorescent dye rhodamine123. We found that type-I acini of the salivary glands, but not type II and III, are responsible for water uptake. In addition, the ingestion of water through the midgut was also observed. Injection or feeding of ouabain, a Na/K-ATPase inhibitor, suppressed water absorption in type I acini. When I. scapularis was offered a droplet of water, ticks rarely imbibed water directly (5%), while some approached the water droplet to use the high humidity formed in the vicinity of the droplet (23%). We conclude that during both on- and off-host stages, type I acini in salivary glands of female Ixodes scapularis absorb water and ions.
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影响因子：
3.8
作者：
Simo, Ladislav;Koci, Juraj;Park, Yoonseong
通讯作者：
Park, Yoonseong
影响因子：
2.4
作者：
LEES, AD
通讯作者：
LEES, AD
影响因子：
2.2
作者：
DUNBAR, BS;WINSTON, PW
通讯作者：
WINSTON, PW
影响因子：
5.8
作者：
JANCIS, EM;CHEN, HX;DANNIES, PS
通讯作者：
DANNIES, PS
DOI：
10.1016/0020-7322(79)90007-2
发表时间：
1979-01-01
期刊：
INTERNATIONAL JOURNAL OF INSECT MORPHOLOGY & EMBRYOLOGY
影响因子：
--
作者：
MEGAW, MJW;BEADLE, DJ
通讯作者：
BEADLE, DJ