Changes of transcriptome and proteome are associated with the enhanced post-anthesis high temperature tolerance induced by pre-anthesis heat priming in wheat
Changes of transcriptome and proteome are associated with the enhanced post-anthesis high temperature tolerance induced by pre-anthesis heat priming in wheat
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转录组和蛋白质组的变化与小麦花前热启动诱导的花后高温耐受性增强有关
DOI：
10.1007/s10725-015-0119-x
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发表时间：
2016-06
影响因子：
4.2
通讯作者：
Jiang Dong
中科院分区：
文献类型：
--
作者：
Xin Caiyun;Wang Xiao;Cai Jian;Zhou Qin;Liu Fulai;Dai Tingbo;Cao Weixing;Jiang Dong
本研究旨在探讨小麦（Triticum aestivumL.）花前热诱导增强花后高温胁迫耐受性的机制。利用Affymetrix小麦基因组芯片分析了花前热诱导和花后高温胁迫后叶片的全基因组基因表达谱，利用二维电泳和MALDI TOF/TOF分析了蛋白质组学。生理分析表明，处理后植株的光合速率和抗氧化酶活性较高，细胞膜氧化损伤较低，表明处理后植株受到的高温伤害较小。花前和花后高温胁迫均调控了88个基因探针和8个蛋白点，且这些探针和蛋白在引物和未引物中表达不同。转录组和蛋白质组分析显示，编码传感和信号、热休克蛋白、氧化还原稳态的基因上调，编码代谢的基因下调。花前热诱导植株与未热诱导植株相比，这种上调和下调可能在应对花后高温胁迫方面发挥保护作用。综上所述，花前热启动可以在转录组和蛋白质组水平上启动驯化反应，从而提高小麦植株后期的耐热性。这些结果对于理解未来气候变化情景下多重热胁迫对小麦作物生产的影响具有重要意义。
This study was to explore the mechanism of the enhanced tolerance to post-anthesis high temperature stress induced by pre-anthesis heat priming in wheat (Triticum aestivumL.). Genome-wide gene expression profiles by Affymetrix Wheat Genome Chip and proteome analysis by 2D electrophoresis and MALDI TOF/TOF were performed in the leaf after pre-anthesis heat priming and post-anthesis high temperature stress. Physiological analyses indicated that primed plants showed higher rates of photosynthesis, activities of antioxidant enzymes and lower cell membrane oxidative damage, suggesting a less high temperature damage in the primed plants. 88 gene probes and 8 protein spots were regulated after both pre-anthesis heat priming and post-anthesis high temperature stress, and the probes and proteins were expressed differently in primed plants from those in non-primed plants. Transcriptome and proteome analyses revealed up-regulation of the genes that encoded sensing and signaling, heat shock proteins, redox homeostasis, and down-regulation of the genes that encoded metabolism. The up-regulation and down-regulation might play protective roles in coping with the post-anthesis high temperature stress in the pre-anthesis heat primed plants compared with non-primed plants. It is concluded that pre-anthesis heat priming could initiate the acclimation responses at both transcriptome and proteome levels for enhancing heat tolerance at later stages in wheat plants. These results are of primary importance for understanding the effects of multi-heat stress on production of wheat crops in future climate change scenarios.
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影响因子：
7.2
作者：
Ton, Jurriaan;D'Alessandro, Marco;Turlings, Ted C. J.
通讯作者：
Turlings, Ted C. J.
影响因子：
7
作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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