Combining Next Generation Sequencing with Bulked Segregant Analysis to Fine Map a Stem Moisture Locus in Sorghum (Sorghum bicolor L. Moench).
Combining Next Generation Sequencing with Bulked Segregant Analysis to Fine Map a Stem Moisture Locus in Sorghum (Sorghum bicolor L. Moench).
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DOI：
10.1371/journal.pone.0127065
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Liu G
中科院分区：
文献类型：
--
作者：
Han Y;Lv P;Hou S;Li S;Ji G;Ma X;Du R;Liu G
高粱是最有前途的生物能源作物之一。甜高粱的茎汁产量和茎糖浓度共同决定了糖产量。群体分离分析（BSA）是一种基因定位技术，用于鉴定含有影响感兴趣性状的遗传基因座的基因组区域，当与深度测序结合时，可以有效地加速基因定位过程。本研究利用QTL分析和BSA与深度测序技术相结合的方法，对一个干秆高粱地方品种的茎水分控制基因qSW 6进行了精细定位。结果表明：（1）高粱品种吉良2号开花前茎含水量在80%左右;灌浆期下降到75%左右，不同节间间差异不大。长白猪G21的茎含水量在剑叶期以后持续下降。从开花期的71%下降到灌浆期的60%。不同节间间差异较大，面团期第7、8节间差异最小（51%）。(ii)控制茎含水量的QTL定位在第6染色体上，位于SSR标记Ch 6 -2和gpsb 069之间，分别解释了第5 ~ 10节间表型变异的34.7-56.9%。(iii)BSA和深度测序分析将相关区域缩小到339 kb，包含38个推定基因。研究结果有助于揭示高粱出汁率的分子机制，从而为提高高粱总糖产量提供理论依据。
Sorghum is one of the most promising bioenergy crops. Stem juice yield, together with stem sugar concentration, determines sugar yield in sweet sorghum. Bulked segregant analysis (BSA) is a gene mapping technique for identifying genomic regions containing genetic loci affecting a trait of interest that when combined with deep sequencing could effectively accelerate the gene mapping process. In this study, a dry stem sorghum landrace was characterized and the stem water controlling locus, qSW6, was fine mapped using QTL analysis and the combined BSA and deep sequencing technologies. Results showed that: (i) In sorghum variety Jiliang 2, stem water content was around 80% before flowering stage. It dropped to 75% during grain filling with little difference between different internodes. In landrace G21, stem water content keeps dropping after the flag leaf stage. The drop from 71% at flowering time progressed to 60% at grain filling time. Large differences exist between different internodes with the lowest (51%) at the 7th and 8th internodes at dough stage. (ii) A quantitative trait locus (QTL) controlling stem water content mapped on chromosome 6 between SSR markers Ch6-2 and gpsb069 explained about 34.7-56.9% of the phenotypic variation for the 5th to 10th internodes, respectively. (iii) BSA and deep sequencing analysis narrowed the associated region to 339 kb containing 38 putative genes. The results could help reveal molecular mechanisms underlying juice yield of sorghum and thus to improve total sugar yield.
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