An In Vitro Small Intestine Model Incorporating a Food Matrix and Bacterial Mock Community for Intestinal Function Testing.
An In Vitro Small Intestine Model Incorporating a Food Matrix and Bacterial Mock Community for Intestinal Function Testing.
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用于肠功能测试的食品基质和细菌模拟群落的体外小肠模型。
DOI：
10.3390/microorganisms11061419
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发表时间：
2023-05-27
期刊：
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
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作者：
消耗的食物通过胃肠道到达小肠，在那里它与微生物群相互作用，与饮食成分形成复杂的关系。在这里，我们提出了一个复杂的体外细胞培养模型的小肠，包括人类细胞，消化，模拟餐，和微生物代表的细菌群落组成的大肠杆菌。coli，L. rhamnosus、S. salivarius，B. B. bifidum和E.粪便该模型用于确定食品级二氧化钛纳米颗粒（TiO2 NPs）（一种常见的食品添加剂）对上皮通透性、肠碱性磷酸酶活性和营养物质跨上皮转运的影响。生理相关浓度的TiO 2对肠道通透性没有影响，但作为食物模型的一部分，会导致甘油三酯转运增加，而在细菌存在的情况下，这种情况会逆转。单个细菌物种对葡萄糖转运没有影响，但细菌群落增加了葡萄糖转运，表明细菌在群落中的行为发生了变化。粘液层内的细菌截留减少与TiO 2暴露，这可能是由于减少粘液层厚度。人类细胞、合成膳食和细菌模拟群落的结合为了解营养变化对小肠功能（包括微生物群）的影响提供了机会。
Consumed food travels through the gastrointestinal tract to reach the small intestine, where it interacts with the microbiota, forming a complex relationship with the dietary components. Here we present a complex in vitro cell culture model of the small intestine that includes human cells, digestion, a simulated meal, and a microbiota represented by a bacterial community consisting of E. coli, L. rhamnosus, S. salivarius, B. bifidum, and E. faecalis. This model was used to determine the effects of food-grade titanium dioxide nanoparticles (TiO2 NPs), a common food additive, on epithelial permeability, intestinal alkaline phosphatase activity, and nutrient transport across the epithelium. Physiologically relevant concentrations of TiO2 had no effect on intestinal permeability but caused an increase in triglyceride transport as part of the food model, which was reversed in the presence of bacteria. Individual bacterial species had no effect on glucose transport, but the bacterial community increased glucose transport, suggesting a change in bacterial behavior when in a community. Bacterial entrapment within the mucus layer was reduced with TiO2 exposure, which may be due to decreased mucus layer thickness. The combination of human cells, a synthetic meal, and a bacterial mock community provides an opportunity to understand the implications of nutritional changes on small intestinal function, including the microbiota.
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影响因子：
2.5
作者：
Ersoz, Nurdan;Canga, Emine Merve;Dudak, Fahriye Ceyda
通讯作者：
Dudak, Fahriye Ceyda
影响因子：
17.3
作者：
GLYNN, J
通讯作者：
GLYNN, J
影响因子：
24.5
作者：
CHACKO, A;CUMMINGS, JH
通讯作者：
CUMMINGS, JH
鼠李糖乳杆菌 GG 治疗可改善脓毒症实验模型中的肠道通透性并调节微生物群失调
影响因子：
5.4
作者：
Chen, Lufang;Li, Hanyu;Yang, Yunmei
通讯作者：
Yang, Yunmei
DOI：
10.1016/j.fct.2019.110896
发表时间：
2020-01
期刊：
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
影响因子：
--
作者：
Kolba N;Guo Z;Olivas FM;Mahler GJ;Tako E
通讯作者：
Tako E