Intra-amniotic administration (Gallus gallus) of TiO(2), SiO(2), and ZnO nanoparticles affect brush border membrane functionality and alters gut microflora populations.
Intra-amniotic administration (Gallus gallus) of TiO(2), SiO(2), and ZnO nanoparticles affect brush border membrane functionality and alters gut microflora populations.
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DOI：
10.1016/j.fct.2019.110896
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发表时间：
2020-01
期刊：
影响因子：
--
通讯作者：
Tako E
中科院分区：
文献类型：
--
作者：
Kolba N;Guo Z;Olivas FM;Mahler GJ;Tako E
金属氧化物纳米颗粒（NP）越来越多地用于食品和农业行业，使人类消费几乎不可避免。本研究的目的是使用原鸡（肉鸡）羊膜内给药生理相关浓度的TiO 2，SiO 2和ZnO，以更好地了解NP暴露对肠道健康和功能的影响。孵化后立即收集血液、盲肠和小肠，以评估铁（Fe）代谢、锌（Zn）代谢、刷状缘膜（BBM）功能和促炎相关蛋白基因表达;血液Fe和Zn水平;盲肠重量;以及肠道微生物菌群的相对丰度。NP类型、剂量和矿物质的存在或不存在显示导致改变的矿物质转运蛋白、BBM功能和促炎基因表达。金属氧化物NP也改变了肠道细菌种群的丰度。总体而言，数据表明体内结果与体外研究一致，NP有可能对肠道功能和健康产生负面影响。
Metal oxide nanoparticles (NP) are increasingly used in the food and agriculture industries, making human consumption nearly unavoidable. The goal of this study was to use the Gallus gallus (broiler chicken) intra-amniotic administration of physiologically relevant concentrations of TiO2, SiO2, and ZnO to better understand the effects of NP exposure on gut health and function. Immediately after hatch, blood, cecum, and small intestine were collected for assessment of iron (Fe)-metabolism, zinc (Zn)-metabolism, brush border membrane (BBM) functional, and pro-inflammatory related proteins gene expression; blood Fe and Zn levels; cecum weight; and the relative abundance of intestinal microflora. NP type, dose, and the presence or absence of minerals was shown to result in altered mineral transporter, BBM functional, and pro-inflammatory gene expression. Metal oxide NP also altered the abundance of intestinal bacterial populations. Overall, the data suggest that the in vivo results align with in vitro studies, and that NP have the potential to negatively affect intestinal functionality and health.
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影响因子：
4.3
作者：
Moreno-Olivas, Fabiola;Tako, Elad;Mahler, Gretchen J.
通讯作者：
Mahler, Gretchen J.
影响因子：
7
作者：
Lomer, MCE;Thompson, RPH;Powell, JJ
通讯作者：
Powell, JJ
DOI：
10.1007/bf00340589
发表时间：
1971-01-01
期刊：
ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE
影响因子：
--
作者：
GUSTAFSSON, BE;MAUNSBACH, AB
通讯作者：
MAUNSBACH, AB
影响因子：
5
作者：
Guo Z;Martucci NJ;Liu Y;Yoo E;Tako E;Mahler GJ
通讯作者：
Mahler GJ
影响因子：
3.5
作者：
Michalczyk, Agnes A.;Ackland, M. Leigh
通讯作者：
Ackland, M. Leigh