Endometrial Stem/Progenitor Cells-Their Role in Endometrial Repair and Regeneration.
Endometrial Stem/Progenitor Cells-Their Role in Endometrial Repair and Regeneration.
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DOI：
10.3389/frph.2021.811537
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发表时间：
2021
影响因子：
--
通讯作者：
Gargett, Caroline E.
中科院分区：
文献类型：
--
作者：
Cousins, Fiona L.;Filby, Caitlin E.;Gargett, Caroline E.
人的子宫内膜是一种非凡的组织，在女性的生殖寿命中经历了大约450个周期的增殖、分化、脱落(月经)、修复和再生。月经后修复是一个极其迅速和无疤痕的过程，腔上皮的再上皮化在脱落开始后48小时内完成。月经后，在循环雌激素水平上升的影响下，在增生期，功能肌从残留的基底层生长。子宫内膜的再生能力归因于干细胞/祖细胞，它们同时存在于基底层的上皮室和基质细胞室。寻找子宫内膜上皮祖细胞(EEPC)的明确标记物已被证明是困难的。一些不同的标记被认为是干细胞的前体标记，包括N-钙粘蛋白、SSEA-1、AXIN2、SOX-9和ALDH1A1，其中一些在体外实验中显示出功能性干细胞活性。每个标记物在腺上皮中都有一个独特的位置(S)，这导致了存在一个分化层次，从基底部的上皮腺的底部到排列在功能基底部的腔上皮，在那里，上皮细胞在分化时表达不同的标记物组合，并沿着腺体向上移动到远离基底骨的功能基底部。血管周围子宫内膜间充质干细胞(EMSCs)可以通过PDGFRCD146和β的共同表达或通过单个标志物SuSD2进行鉴定。这篇综述将详细介绍已知的子宫内膜干/祖标志物；它们在月经周期中的身份、位置、已知的相互作用和层次结构，特别是月经后修复和雌激素驱动的再生，以及它们在月经相关疾病(如子宫内膜异位症和再生相关疾病Asherman综合征)中的可能作用。我们还将重点介绍能够更好地理解干细胞/祖细胞在修复和再生中的作用的新技术，包括3D器官、3D切片培养和单细胞水平的基因测序。由于小鼠模型通常用于研究月经、修复和再生，我们还将详细介绍已在体内研究的小鼠干细胞/祖细胞标志物。
The human endometrium is a remarkable tissue, undergoing ~450 cycles of proliferation, differentiation, shedding (menstruation), repair, and regeneration over a woman's reproductive lifespan. Post-menstrual repair is an extremely rapid and scar-free process, with re-epithelialization of the luminal epithelium completed within 48 h of initiation of shedding. Following menstruation, the functionalis grows from the residual basalis layer during the proliferative phase under the influence of rising circulating estrogen levels. The regenerative capacity of the endometrium is attributed to stem/progenitor cells which reside in both the epithelial and stromal cell compartments of the basalis layer. Finding a definitive marker for endometrial epithelial progenitors (eEPCs) has proven difficult. A number of different markers have been suggested as putative progenitor markers including, N-cadherin, SSEA-1, AXIN2, SOX-9 and ALDH1A1, some of which show functional stem cell activity in in vitro assays. Each marker has a unique location(s) in the glandular epithelium, which has led to the suggestion that a differentiation hierarchy exists, from the base of epithelial glands in the basalis to the luminal epithelium lining the functionalis, where epithelial cells express different combinations of markers as they differentiate and move up the gland into the functionalis away from the basalis niche. Perivascular endometrial mesenchymal stem cells (eMSCs) can be identified by co-expression of PDGFRβ and CD146 or by a single marker, SUSD2. This review will detail the known endometrial stem/progenitor markers; their identity, location and known interactions and hierarchy across the menstrual cycle, in particular post-menstrual repair and estrogen-driven regeneration, as well as their possible contributions to menstruation-related disorders such as endometriosis and regeneration-related disorder Asherman's syndrome. We will also highlight new techniques that allow for a greater understanding of stem/progenitor cells' role in repair and regeneration, including 3D organoids, 3D slice cultures and gene sequencing at the single cell level. Since mouse models are commonly used to study menstruation, repair and regeneration we will also detail the mouse stem/progenitor markers that have been investigated in vivo.
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影响因子：
4
作者：
Cousins, Fiona L.;Dorien, F. O.;Gargett, Caroline E.
通讯作者：
Gargett, Caroline E.
影响因子：
3.7
作者：
Cousins FL;Murray A;Esnal A;Gibson DA;Critchley HO;Saunders PT
通讯作者：
Saunders PT
影响因子：
4.6
作者：
Boretto, Matteo;Cox, Benoit;Vankelecom, Hugo
通讯作者：
Vankelecom, Hugo
影响因子：
3.6
作者：
Bailey, Amelia P.;Hill, Abby S.;Missmer, Stacey A.
通讯作者：
Missmer, Stacey A.
DOI：
10.1039/c6ib00245e
发表时间：
2017-04-18
期刊：
Integrative biology : quantitative biosciences from nano to macro
影响因子：
--
作者：
Cook CD;Hill AS;Guo M;Stockdale L;Papps JP;Isaacson KB;Lauffenburger DA;Griffith LG
通讯作者：
Griffith LG