Regulatory effects of cAMP receptor protein (CRP) on porin genes and its own gene in Yersinia pestis.
Regulatory effects of cAMP receptor protein (CRP) on porin genes and its own gene in Yersinia pestis.
复制标题
cAMP受体蛋白(CRP)对鼠疫耶尔森氏菌孔蛋白基因及其自身基因的调控作用
DOI：
10.1186/1471-2180-11-40
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发表时间：
2011-02-23
期刊：
影响因子：
4.2
通讯作者：
Yang R
中科院分区：
文献类型：
--
作者：
Gao H;Zhang Y;Yang L;Liu X;Guo Z;Tan Y;Han Y;Huang X;Zhou D;Yang R
背景
cAMP受体蛋白（CRP）是一种全局性细菌调节因子，控制许多靶基因。CRP-cAMP复合物调节E.大肠杆菌直接作用于ompR-envZ，涉及多个靶启动子的正调控和负调控;此外，它通过直接作用于ompR-envZ间接控制孔蛋白的产生。CRP的自动调节也已在E.杆菌然而，CRP对孔蛋白基因及其自身基因的调控在Y.鼠疫
结果
Y.鼠疫采用一种独特的机制，表明CRP对ompR-envZ操纵子没有调节作用;然而，它直接刺激ompC和ompF，同时抑制ompX。在Y染色体上未检测到CRP与自身基因之间的转录调控关系。这也与CRP在大肠杆菌中既是自身基因的抑制物又是激活物的事实相反。杆菌很可能Y.鼠疫菌OmpR和CRP分别感知不同的信号（中等渗透压和细胞cAMP水平）以独立地调节孔蛋白基因。
结论
虽然Y. pestis与E.大肠杆菌，CRP识别的共有DNA序列被两种细菌共享，Y.鼠疫菌CRP不识别自身基因启动子，而直接识别ompC、F和X基因启动子，这与大肠杆菌的相关调控回路不同。杆菌这里提出的数据表明，显着的重塑CRP介导的调节孔蛋白基因和自己的一个这两种细菌之间。
Background
The cAMP receptor protein (CRP) is a global bacterial regulator that controls many target genes. The CRP-cAMP complex regulates the ompR-envZ operon in E. coli directly, involving both positive and negative regulations of multiple target promoters; further, it controls the production of porins indirectly through its direct action on ompR-envZ. Auto-regulation of CRP has also been established in E. coli. However, the regulation of porin genes and its own gene by CRP remains unclear in Y. pestis.
Results
Y. pestis employs a distinct mechanism indicating that CRP has no regulatory effect on the ompR-envZ operon; however, it stimulates ompC and ompF directly, while repressing ompX. No transcriptional regulatory association between CRP and its own gene can be detected in Y. pestis, which is also in contrast to the fact that CRP acts as both repressor and activator for its own gene in E. coli. It is likely that Y. pestis OmpR and CRP respectively sense different signals (medium osmolarity, and cellular cAMP levels) to regulate porin genes independently.
Conclusion
Although the CRP of Y. pestis shows a very high homology to that of E. coli, and the consensus DNA sequence recognized by CRP is shared by the two bacteria, the Y. pestis CRP can recognize the promoters of ompC, F, and X directly rather than that of its own gene, which is different from the relevant regulatory circuit of E. coli. Data presented here indicate a remarkable remodeling of the CRP-mediated regulation of porin genes and of its own one between these two bacteria.
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