Culture-free bacterial detection and identification from blood with rapid, phenotypic, antibiotic susceptibility testing.
Culture-free bacterial detection and identification from blood with rapid, phenotypic, antibiotic susceptibility testing.
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DOI：
10.1038/s41598-018-21520-9
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发表时间：
2018-02-21
影响因子：
4.6
通讯作者：
Yau ST
中科院分区：
文献类型：
--
作者：
Shi X;Kadiyala U;VanEpps JS;Yau ST
目前以培养为基础的诊断血流感染的方法与及时治疗和遏制多药耐药菌(MDRO)的流行不相称，因为它需要很长的时间才能取得结果。血液感染通常涉及极低的细菌浓度(例如，10个菌落形成单位/毫升)，这需要一个劳动密集型过程和长达72 小时的诊断。在这里，我们展示了一种无培养的方法来实现对血流感染的快速诊断。为实现感染的超灵敏、快速检测、鉴定(ID)和药敏试验(AST)，研制了内源信号电流放大的免疫检测平台。该平台具有监测血液中短期(1-2 小时)细菌生长的能力，能够提供84分钟的同步检测和对低于10cfu/ml水平的血液样本的ID和204分钟的AST。表现出对AST敏感-中抗的能力。
The current culture-based approach for the diagnosis of bloodstreams infection is incommensurate with timely treatment and curbing the prevalence of multi-drug resistant organisms (MDROs) due to its long time-to-result. Bloodstream infections typically involve extremely low (e.g., <10 colony-forming unit (CFU)/mL) bacterial concentrations that require a labor-intensive process and as much as 72 hours to yield a diagnosis. Here, we demonstrate a culture-free approach to achieve rapid diagnosis of bloodstream infections. An immuno-detection platform with intrinsic signal current amplification was developed for the ultrasensitive, rapid detection, identification (ID) and antibiotic susceptibility testing (AST) of infections. With its capability of monitoring short-term (1–2 hours) bacterial growth in blood, the platform is able to provide 84-minute simultaneous detection and ID in blood samples below the 10 CFU/mL level and 204-minute AST. The susceptible-intermediate-resistant AST capacity was demonstrated.
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影响因子：
9.4
作者：
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