RNA Interference by Cyanobacterial Feeding Demonstrates the SCSG1 Gene Is Essential for Ciliogenesis during Oral Apparatus Regeneration in Stentor.
RNA Interference by Cyanobacterial Feeding Demonstrates the SCSG1 Gene Is Essential for Ciliogenesis during Oral Apparatus Regeneration in Stentor.
复制标题
蓝藻喂养的 RNA 干扰表明 SCSG1 基因对于 Stentor 口腔器具再生过程中纤毛发生至关重要
DOI：
10.3390/microorganisms9010176
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发表时间：
2021-01-15
期刊：
影响因子：
4.5
通讯作者：
Xiong J
中科院分区：
文献类型：
--
作者：
Wei W;Jiang C;Chai X;Zhang J;Zhang CC;Miao W;Xiong J
在巨大的纤毛虫Stentorcoeruleus，口腔器官（OA）的再生是一个实验上容易处理的再生范例，发生通过一系列的形态步骤。OA再生被认为是由一个复杂的调控系统驱动的，该系统协调保守和特定基因的时间表达。我们以前发现了一个S。蓝绿色特异性基因（命名为SCSG 1）在OA再生的纤毛发生阶段显著上调，在第一次OA纤毛出现的阶段具有表达高峰。以蓝藻集胞藻6803为研究对象，建立了一种新的RNA干扰方法。蓝核该方法可显著降低SCSG 1基因的表达，诱导S.表明SCSG 1在S.蓝核这种利用蓝藻取食的RNA干扰方法对其他纤毛虫的研究具有潜在的应用价值。
In the giant ciliate Stentor coeruleus, oral apparatus (OA) regeneration is an experimentally tractable regeneration paradigm that occurs via a series of morphological steps. OA regeneration is thought to be driven by a complex regulatory system that orchestrates the temporal expression of conserved and specific genes. We previously identified a S. coeruleus-specific gene (named SCSG1) that was significantly upregulated during the ciliogenesis stages of OA regeneration, with an expression peak at the stage of the first OA cilia appearance. We established a novel RNA interference (RNAi) method through cyanobacteria Synechocystis sp. PCC6803 feeding in S. coeruleus. The expression of SCSG1 gene was significantly knocked down by using this method and induced abnormal ciliogenesis of OA regeneration in S. coeruleus, suggesting that SCSG1 is essential for OA regeneration in S. coeruleus. This novel RNAi method by cyanobacterial feeding has potential utility for studying other ciliates.
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