Biocompatible High-Resolution 3D-Printed Microfluidic Devices: Integrated Cell Chemotaxis Demonstration.
Biocompatible High-Resolution 3D-Printed Microfluidic Devices: Integrated Cell Chemotaxis Demonstration.
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生物相容性高分辨率 3D 打印微流体器件：集成细胞趋化性演示。
DOI：
10.3390/mi14081589
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发表时间：
2023-08-12
期刊：
影响因子：
3.4
通讯作者：
中科院分区：
文献类型：
--
作者：
我们展示了一种使用基于阿伏苯宗的生物相容性树脂作为UV吸收剂有效3D打印具有高分辨率特征的微流体设备的方法。我们的方法依赖于对3D打印机源光谱进行光谱整形，使其与阿伏苯宗的吸收光谱完全重叠。完全重叠对于有效地限制光学穿透深度是必不可少的，这是实现高平面外分辨率所需的。我们通过在微流体腔室中3D打印15 m方形柱来证明实践中的高分辨率，其中柱间隔7.7 m并打印有5 m层。此外，我们使用生物相容性树脂显示可靠的膜阀和泵。瓣膜经1，000，000次驱动试验，未观察到性能下降。最后，我们创建了一个浓度梯度生成（CG）组件，并利用它在两个设备设计的细胞趋化性研究。第一种设计依赖于外部双注射器泵来产生源和汇流以供应CG通道，而第二种设计是一个完整的集成装置，包括芯片上的泵、阀和储液器。两种器械类型均接种了经过化学引诱物CG处理的贴壁细胞，并且均显示出趋化性细胞迁移的明确证据。此外，集成器械的细胞迁移与外部注射泵器械相当。该演示说明了我们的集成趋化性测定方法和高分辨率生物相容性树脂3D打印制造工艺的有效性。此外，我们的3D打印工艺已经过调整，以实现快速制造，因为两种设备设计的打印时间分别为8分钟和15分钟。
We demonstrate a method to effectively 3D print microfluidic devices with high-resolution features using a biocompatible resin based on avobenzone as the UV absorber. Our method relies on spectrally shaping the 3D printer source spectrum so that it is fully overlapped by avobenzone’s absorption spectrum. Complete overlap is essential to effectively limit the optical penetration depth, which is required to achieve high out-of-plane resolution. We demonstrate the high resolution in practice by 3D printing 15 m square pillars in a microfluidic chamber, where the pillars are separated by 7.7 m and are printed with 5 m layers. Furthermore, we show reliable membrane valves and pumps using the biocompatible resin. Valves are tested to 1,000,000 actuations with no observable degradation in performance. Finally, we create a concentration gradient generation (CG) component and utilize it in two device designs for cell chemotaxis studies. The first design relies on an external dual syringe pump to generate source and sink flows to supply the CG channel, while the second is a complete integrated device incorporating on-chip pumps, valves, and reservoirs. Both device types are seeded with adherent cells that are subjected to a chemoattractant CG, and both show clear evidence of chemotactic cellular migration. Moreover, the integrated device demonstrates cellular migration comparable to the external syringe pump device. This demonstration illustrates the effectiveness of our integrated chemotactic assay approach and high-resolution biocompatible resin 3D printing fabrication process. In addition, our 3D printing process has been tuned for rapid fabrication, as printing times for the two device designs are, respectively, 8 and 15 min.
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影响因子：
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