Long Non-Coding RNA Taurine Upregulated Gene 1 Affects Cell Apoptosis and Cell Cycle through Downregulating Cell Division Control Protein 42 Homolog in Osteoblasts
Long Non-Coding RNA Taurine Upregulated Gene 1 Affects Cell Apoptosis and Cell Cycle through Downregulating Cell Division Control Protein 42 Homolog in Osteoblasts
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长非编码 RNA 牛磺酸上调基因 1 通过下调成骨细胞中细胞分裂控制蛋白 42 同源物影响细胞凋亡和细胞周期
DOI：
10.36468/pharmaceutical-sciences.spl.242
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发表时间：
2021
影响因子：
0.5
通讯作者：
Jiang Qian
中科院分区：
文献类型：
--
作者：
Cao Yiting;Wu Y.;Gu Q.;Fang Fang;Jiang Qian
探讨长链非编码核糖核酸牛磺酸上调基因1对成骨细胞凋亡和细胞周期的调控及其潜在机制是本研究的主要目的。利用过表达质粒和小干扰核糖核酸调控成骨细胞中牛磺酸上调基因1的表达。定量反转录聚合酶链反应检测牛磺酸上调基因1的表达，流式细胞术检测牛磺酸上调基因1对成骨细胞凋亡和细胞周期的影响。采用定量反转录聚合酶链反应和western blot分析检测牛磺酸上调基因1对细胞分裂控制蛋白42同源物的调控作用，并结合功能恢复实验验证牛磺酸上调基因1通过细胞分裂控制蛋白42同源物调控成骨细胞凋亡和细胞周期。沉默牛磺酸上调基因1可促进成骨细胞凋亡，阻止成骨细胞进入生长2期/有丝分裂期。过表达牛磺酸上调基因1对成骨细胞凋亡和细胞周期无明显影响。沉默牛磺酸上调基因1抑制细胞分裂控制蛋白42同源物的表达，过表达牛磺酸上调基因1促进细胞分裂控制蛋白42同源物的表达。此外，与牛磺酸上调基因1过表达组相比，牛磺酸上调基因1过表达+细胞分裂控制蛋白42同源拮抗剂组成骨细胞凋亡明显增加。与牛磺酸上调基因1沉默组相比，牛磺酸上调基因1沉默+细胞分裂控制蛋白42同源激动剂组成骨细胞凋亡明显减少，进入生长2期/有丝分裂期的成骨细胞数量明显增加。沉默牛磺酸上调基因1可通过下调细胞分裂控制蛋白42同源物的表达，促进成骨细胞凋亡，阻止成骨细胞进入生长2期/有丝分裂期。牛磺酸上调基因1的正常表达水平通过稳定细胞分裂控制蛋白42同源物的表达来维持成骨细胞的正常凋亡和周期。
To explore the regulation and potential mechanism of long non-coding ribonucleic acid taurine upregulated gene 1 on the apoptosis and cell cycle of osteoblasts is the main purpose. The overexpression plasmid and small interfering ribonucleic acid were used to regulate the expression of taurine upregulated gene 1 in osteoblasts. The expression of taurine upregulated gene 1 was detected by quantitative reverse transcription polymerase chain reaction and the effect of taurine upregulated gene 1 on the apoptosis and cell cycle of osteoblasts were detected by flow cytometry, quantitative reverse transcription polymerase chain reaction and western blot analyses were used to detect the regulation of taurine upregulated gene 1 on cell division control protein 42 homolog and combined with functional recovery experiments to verify that taurine upregulated gene 1 regulated the apoptosis and cell cycle of osteoblasts through cell division control protein 42 homolog. Silencing taurine upregulated gene 1 promoted the apoptosis of osteoblasts and blocked osteoblasts from entering the growth 2 phase/mitotic phase. Overexpression of taurine upregulated gene 1 had no significant effect on the apoptosis and cell cycle of osteoblasts. Silencing taurine upregulated gene 1 inhibited the expression of cell division control protein 42 homolog and overexpression of taurine upregulated gene 1 promoted the expression of cell division control protein 42 homolog. In addition, compared with the taurine upregulated gene 1 overexpression group, the apoptosis of osteoblasts in the taurine upregulated gene 1 overexpression+cell division control protein 42 homolog antagonist group increased significantly. Compared with the taurine upregulated gene 1 silencing group, the apoptosis of osteoblasts in the taurine upregulated gene 1 silencing+cell division control protein 42 homolog agonist group was significantly reduced and the number of osteoblasts entering the growth 2 phase/mitotic phase was significantly increased. Silencing taurine upregulated gene 1 promotes the apoptosis of osteoblasts and prevented osteoblasts from entering growth 2 phase/mitotic phase by downregulating the expression of cell division control protein 42 homolog. The normal expression level of taurine upregulated gene 1 maintains the normal apoptosis and cycle of osteoblasts by stabilizing the expression of cell division control protein 42 homolog.
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