Pulmonary Arterial Hypertension Affects the Rat Gut Microbiome.
Pulmonary Arterial Hypertension Affects the Rat Gut Microbiome.
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DOI：
10.1038/s41598-018-27682-w
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发表时间：
2018-06-26
影响因子：
4.6
通讯作者：
Perez-Vizcaino F
中科院分区：
文献类型：
--
作者：
Callejo M;Mondejar-Parreño G;Barreira B;Izquierdo-Garcia JL;Morales-Cano D;Esquivel-Ruiz S;Moreno L;Cogolludo Á;Duarte J;Perez-Vizcaino F
我们分析了肺动脉高压（PAH）是否会改变大鼠粪便微生物群。 Wistar大鼠注射VEGF受体拮抗剂SU5416（20mg/kg s.c.），并在缺氧（10％O2，PAH）下持续两周，或注射媒介物并在常氧条件下（对照）。获取粪便样本，并通过 16S rRNA 基因测序和生物信息分析确定微生物组组成。未发现 PAH 对全球微生物群产生影响（α 或 β 多样性）。然而，基于分类学的分析表明，暴露于多环芳烃的大鼠表现出肠道菌群失调。具体而言，PAH 大鼠的厚壁菌门与拟杆菌门的比例增加了三倍。在厚壁菌门内，PAH 中细菌家族的相对丰度没有大的变化。在拟杆菌门中，所有科的 PAH 含量均较低。根据产生短链脂肪酸的细菌属，在对照和 PAH 簇之间观察到明显的分离。此外，PAH 大鼠血清中的乙酸盐减少。总之，PAH 会改变粪便微生物群的组成。这种不平衡的细菌生态系统可能通过改变免疫、激素和代谢稳态而在 PAH 中发挥病理生理作用。
We have analysed whether pulmonary arterial hypertension (PAH) alters the rat faecal microbiota. Wistar rats were injected with the VEGF receptor antagonist SU5416 (20 mg/kg s.c.) and followed for 2 weeks kept in hypoxia (10% O2, PAH) or injected with vehicle and kept in normoxia (controls). Faecal samples were obtained and microbiome composition was determined by 16S rRNA gene sequencing and bioinformatic analysis. No effect of PAH on the global microbiome was found (α- or β-diversity). However, PAH-exposed rats showed gut dysbiosis as indicated by a taxonomy-based analysis. Specifically, PAH rats had a three-fold increase in Firmicutes-to-Bacteroidetes ratio. Within the Firmicutes phylum, there were no large changes in the relative abundance of the bacterial families in PAH. Among Bacteroidetes, all families were less abundant in PAH. A clear separation was observed between the control and PAH clusters based on short chain fatty acid producing bacterial genera. Moreover, acetate was reduced in the serum of PAH rats. In conclusion, faecal microbiota composition is altered as a result of PAH. This misbalanced bacterial ecosystem might in turn play a pathophysiological role in PAH by altering the immunologic, hormonal and metabolic homeostasis.
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影响因子：
5.2
作者：
Liu Z;Liu HY;Zhou H;Zhan Q;Lai W;Zeng Q;Ren H;Xu D
通讯作者：
Xu D
影响因子：
4.6
作者：
López P;de Paz B;Rodríguez-Carrio J;Hevia A;Sánchez B;Margolles A;Suárez A
通讯作者：
Suárez A
DOI：
10.1126/science.1195568
发表时间：
2010-12-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Lee YK;Mazmanian SK
通讯作者：
Mazmanian SK
影响因子：
--
作者：
Fu ZD;Cui JY
通讯作者：
Cui JY
影响因子：
64.8
作者：
Fukuda, Shinji;Toh, Hidehiro;Ohno, Hiroshi
通讯作者：
Ohno, Hiroshi