Combining perfluorocarbon and superparamagnetic iron-oxide cell labeling for improved and expanded applications of cellular MRI.
Combining perfluorocarbon and superparamagnetic iron-oxide cell labeling for improved and expanded applications of cellular MRI.
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DOI：
10.1002/mrm.25120
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发表时间：
2015-01
影响因子：
3.3
通讯作者：
Ho, Chien
中科院分区：
文献类型：
--
作者：
Hitchens, T. Kevin;Liu, Li;Foley, Lesley M.;Simplaceanu, Virgil;Ahrens, Eric T.;Ho, Chien
The ability to detect the migration of cells in living organisms is fundamental in understanding biological processes and important for the development of novel cell-based therapies to treat disease. MRI can be used to detect the migration of cells labeled with superparamagnetic iron-oxide (SPIO) or perfluorocarbon (PFC) agents. In this study, we explored combining these two cell-labeling approaches to overcome current limitations and enable new applications for cellular MRI. We characterized 19F-NMR relaxation properties of PFC-labeled cells in the presence of SPIO and imaged cells both ex vivo and in vivo in a rodent inflammation model to demonstrate selective visualization of cell populations. We show that with UTE3D, RARE and FLASH 19F images one can uniquely identify PFC-labeled cells, co-localized PFC- and SPIO-labeled cells, and PFC/SPIO co-labeled cells. This new methodology has the ability to improve and expand applications of MRI cell tracking. Combining PFC and SPIO strategies can potentially provide a method to quench PFC signal transferred from dead cells to macrophages, thereby eliminating false positives. In addition, combining these techniques could also be used to track two cell types simultaneously and probe cell-cell proximity in vivo with MRI.
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影响因子：
3.1
作者：
Chen, Chih-Lung;Zhang, Haosen;Ye, Qing;Hsieh, Wen-Yuan;Hitchens, T. Kevin;Shen, Hsin-Hsin;Liu, Li;Wu, Yi-Jen;Foley, Lesley M.;Wang, Shian-Jy;Ho, Chien
通讯作者：
Ho, Chien
影响因子：
2.8
作者：
Hong H;Yang Y;Zhang Y;Cai W
通讯作者：
Cai W
用于MRI细胞跟踪的含氟纳米乳液。
DOI：
10.1002/wnan.35
发表时间：
2009-09
影响因子：
8.6
作者：
Janjic, Jelena M.;Ahrens, Eric T.
通讯作者：
Ahrens, Eric T.
影响因子：
3.3
作者：
Ferrauto, Giuseppe;Castelli, Daniela Delli;Aime, Silvio
通讯作者：
Aime, Silvio
影响因子：
5.4
作者：
GIROLOMONI, G;LUTZ, MB;RICCIARDICASTAGNOLI, P
通讯作者：
RICCIARDICASTAGNOLI, P