A new nano-sized iron oxide particle with high sensitivity for cellular magnetic resonance imaging.
A new nano-sized iron oxide particle with high sensitivity for cellular magnetic resonance imaging.
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DOI：
10.1007/s11307-010-0430-x
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发表时间：
2011-10
影响因子：
3.1
通讯作者：
Ho, Chien
中科院分区：
文献类型：
--
作者：
Chen, Chih-Lung;Zhang, Haosen;Ye, Qing;Hsieh, Wen-Yuan;Hitchens, T. Kevin;Shen, Hsin-Hsin;Liu, Li;Wu, Yi-Jen;Foley, Lesley M.;Wang, Shian-Jy;Ho, Chien
在这项研究中，我们研究了由工业技术研究院 (ITRI) 设计的新合成的涂有聚乙二醇 (PEG) 的纳米级氧化铁颗粒 (IOP) 的标记效率和磁共振成像 (MRI) 信号灵敏度。从大鼠体内分离出巨噬细胞、骨髓源性树突状细胞和间充质干细胞 (MSC)，并通过与 ITRI-IOP 一起孵育进行标记，以及其他三种不同尺寸和涂层的氧化铁颗粒作为参考。这些标记的细胞通过透射电子显微镜（TEM）、光学和荧光显微镜、幻影MRI、最后对注入标记巨噬细胞的大鼠移植心脏进行体内MRI和离体磁共振显微镜（MRM）进行表征。 ITRI-IOP 的纵向 (r1) 和横向 (r2) 弛豫率分别为 22.71 和 319.2 s−1 mM−1。 TEM 和显微图像表明所有细胞类型的每个细胞摄取了多个 ITRI-IOP 颗粒。 ITRI-IOP 提供的灵敏度与体模 MRI 中显示的其他三种粒子相当或更高。体内 MRI 和离体 MRM 检测到排斥心脏中的点状低信号点，很可能是由 ITRI-IOP 标记的巨噬细胞积聚引起的。 ITRI-IOP 是纳米级氧化铁颗粒，在细胞标记（包括吞噬细胞和非吞噬细胞）方面表现出高效率。此外，它在 T2* 加权 MRI 中提供了出色的灵敏度，因此可以作为体内细胞 MRI 的有前景的造影剂。
In this study, we investigated the labeling efficiency and magnetic resonance imaging (MRI) signal sensitivity of a newly synthesized, nano-sized iron oxide particle (IOP) coated with polyethylene glycol (PEG), designed by Industrial Technology Research Institute (ITRI). Macrophages, bone-marrow-derived dendritic cells, and mesenchymal stem cells (MSCs) were isolated from rats and labeled by incubating with ITRI-IOP, along with three other iron oxide particles in different sizes and coatings as reference. These labeled cells were characterized with transmission electron microscopy (TEM), light and fluorescence microscopy, phantom MRI, and finally in vivo MRI and ex vivo magnetic resonance microscopy (MRM) of transplanted hearts in rats infused with labeled macrophages. The longitudinal (r1) and transverse (r2) relaxivities of ITRI-IOP are 22.71 and 319.2 s−1 mM−1, respectively. TEM and microscopic images indicate the uptake of multiple ITRI-IOP particles per cell for all cell types. ITRI-IOP provides sensitivity comparable or higher than the other three particles shown in phantom MRI. In vivo MRI and ex vivo MRM detect punctate spots of hypointensity in rejecting hearts, most likely caused by the accumulation of macrophages labeled by ITRI-IOP. ITRI-IOP, the nano-sized iron oxide particle, shows high efficiency in cell labeling, including both phagocytic and non-phagocytic cells. Furthermore, it provides excellent sensitivity in T2*-weighted MRI, and thus can serve as a promising contrast agent for in vivo cellular MRI.
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影响因子：
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