Establishment of AIDS animal model with SIVmac239 infected Chinese rhesus monkey
Establishment of AIDS animal model with SIVmac239 infected Chinese rhesus monkey
复制标题
SIVmac239感染的恒河猴建立艾滋病动物模型
DOI：
10.1007/s12250-007-0053-9
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发表时间：
2007-12
期刊：
影响因子：
--
通讯作者：
Zheng, Yong-tang
中科院分区：
文献类型：
--
作者：
Li, Ming-hua;Wang, Yuan-yuan;Zhang, Gao-hong;Xia, Hou-jun;Li, Shao-you;Wang, Lu;Zheng, Yong-tang
在本研究中，两只中国恒河猴静脉注射了5000 tcid50的SIVmac239。在121天内，定期监测外周血CD4+T淋巴细胞数量、血浆病毒载量、病毒前DNA和病毒体液抗体的变化。在感染早期，在PBMCs中检测到原病毒DNA，并从PBMCs中分离到感染性SIVmac239病毒。与此同时，CD4+T淋巴细胞数量明显下降，并在121天的感染期内维持在较低水平。血浆病毒载量在接种后第2周达到峰值，随后保持稳定。此外，在血浆中检测到针对病毒蛋白的抗体。结果表明，这2只恒河猴成功感染了SIVmac239。该动物模型可用于进一步的艾滋病研究。
In the present research, two Chinese rhesus monkeys were inoculated intravenously with 5000 TCID50of SIVmac239. The changes in the numbers of CD4+T lymphocyte in peripheral blood, plasma viral loads, proviral DNA and humoral antibodies against virus were periodically monitored during 121 days. At the early stage of infection, proviral DNA had been detected in PBMCs, and infectious SIVmac239 virus had been isolated from PBMCs. At the same period, the numbers of CD4+T lymphocytes were significantly decreased, and maintained at low level during the 121-day period of infection. Plasma viral loads reached the peak at week 2 post-inoculation and kept at a steady state subsequently. Moreover, antibodies against viral proteins were detected from plasma. All the results showed that the two Chinese rhesus monkeys had been infected with SIVmac239 successfully. This animal model can be applied for further AIDS researches.
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影响因子：
5.4
作者：
McDermott, AB;Mitchen, J;Watkins, DI
通讯作者：
Watkins, DI
影响因子：
--
作者：
Zheng Yong-tang
通讯作者：
Zheng Yong-tang
影响因子：
0.7
作者：
C. Miller;K. Abel
通讯作者：
C. Miller;K. Abel
影响因子：
5.4
作者：
Abel, K;Compton, L;Miller, CJ
通讯作者：
Miller, CJ
DOI：
--
发表时间：
2005
期刊：
Journal of Guangzhou University of Traditional Chinese Medicine
影响因子：
--
作者：
Fu Linchun
通讯作者：
Fu Linchun