Antibodies to lipopolysaccharides after immunization of humans with the rough mutant Escherichia coli J5.
Antibodies to lipopolysaccharides after immunization of humans with the rough mutant Escherichia coli J5.
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用粗突变型大肠杆菌 J5 免疫人类后产生脂多糖抗体。
DOI：
10.1093/infdis/163.4.769
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
M. Glauser
中科院分区：
文献类型：
--
作者：
J. Baumgartner;D. Heumann;T. Calandra;M. Glauser
为了研究大肠杆菌J5煮熟细胞免疫后是否诱导针对深层核心结构的抗体，我们在免疫前和免疫后2周检测了70名志愿者血清中针对J5脂多糖（LPS）、Re LPS和脂质A的抗体。为了提高ELISA的灵敏度和特异性，采用核心脂多糖与高密度脂蛋白的复合物代替游离核心脂多糖作为抗原。观察到针对J5 LPS的抗体中位数增加了三倍，但针对Re LPS或脂质A的抗体未发现显著增加。由于J5抗血清不与几种光滑LPS或与Re LPS和脂质A发生反应，因此无法证明交叉反应性。因此，志愿者接种大肠杆菌J5产生了针对J5 LPS的适度特异性抗体反应。先前用J5抗血清观察到的保护机制尚不清楚。
To investigate whether immunization with Escherichia coli J5 boiled cells induces antibodies directed at deep core structures, antibodies against J5 lipopolysaccharide (LPS), Re LPS, and lipid A were measured in the serum of 70 volunteers before and 2 weeks after immunization. To improve the sensitivity and the specificity of ELISA, complexes of core LPS with high-density lipoproteins were used instead of free core LPS as antigens. A median three-fold increase in antibodies directed against J5 LPS was observed, but no significant increase in the antibodies against Re LPS or lipid A was found. Since J5 antiserum did not react with several smooth LPS or with Re LPS and lipid A, cross-reactivity could not be demonstrated. Thus, immunization of volunteers with E. coli J5 produced a modest specific antibody response against J5 LPS. The mechanism of protection previously observed with J5 antiserum remains unclear.
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DOI：
10.1073/pnas.82.6.1790
发表时间：
1985
影响因子：
11.1
作者：
Teng,NN;Kaplan,HS;Hebert,JM;Moore,C;Douglas,H;Wunderlich,A;Braude,AI
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期刊：
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DOI：
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影响因子：
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DOI：
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发表时间：
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期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Ziegler,EJ
通讯作者：
Ziegler,EJ