Fabrication and application of a new DNA biosensor based on on-substrate PCR and electrochemistry
Fabrication and application of a new DNA biosensor based on on-substrate PCR and electrochemistry
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基于底物PCR和电化学的新型DNA生物传感器的制备及应用
DOI：
10.1016/j.snb.2011.08.034
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发表时间：
2011-12
影响因子：
8.4
通讯作者：
Kang, Jingwan
中科院分区：
文献类型：
--
作者：
Wu, Guofan;Yang, Ning;Zhang, Tengguo;Wang, Zhihua;Lu, Xiaoquan;Kang, Jingwan
以金电极固定的DNA探针作为金电极上的不对称PCR引物。在不对称PCR过程中，DNA探针在PCR溶液中存在靶链的情况下延伸。PCR后，dsDNA变性，靶DNA被去除，只有延伸的探针保留在AuE上。最后将亚甲基蓝电化学指示剂与延伸探针结合，测定了指示剂的电化学信号。此信号高于仅由原始探针修饰的AuE的信号。当PCR溶液中没有靶标时，探针不延伸，信号不增加。从4种不同长度的靶标：寡核苷酸、PCR产物、分子克隆载体DNA和转基因辣椒总基因组DNA中成功检测到几丁质酶基因的特异性序列，估计检测限分别为7.3×10−12、3.2×10−11、5.4×10− 11和4.1×10−10moll− 1。对该生物传感器进行了再生实验，结果表明其半衰期为6次。
DNA probes immobilized on a gold electrode (AuE) were employed as the primers of asymmetric PCR on the AuE. In the asymmetric PCR process, the DNA probes extended in the presence of target strands in the PCR solution. After PCR the dsDNAs were denaturalized and the target DNAs were eliminated and only the extended probes maintained on the AuE. At last the electrochemical indicator of methylene blue combined to the extended probes and the electrochemical signal of indicator was measured. This signal was higher than that of the AuE modified only by original probe. When there was no target in the PCR solution, the probe did not extend and the signal did not increase. The specific sequences of chitinase gene were detected successfully from four sorts of target with different length: oligonucleotide acid, PCR products, molecule cloning vector DNA and total genome DNA of transgenic capsicum, and the estimated detection limit were 7.3×10−12, 3.2×10−11, 5.4×10−11and 4.1×10−10moll−1respectively. The regeneration of the biosensor was also tested and the results indicated that its half life was 6 times.
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影响因子：
6.2
作者：
Kerman, K;Ozkan, D;Ozsoz, M
通讯作者：
Ozsoz, M
影响因子：
6.6
作者：
Ningning Zhu;Zhu Chang;P. He;Yu-zhi Fang
通讯作者：
Ningning Zhu;Zhu Chang;P. He;Yu-zhi Fang
DOI：
10.1063/1.3703298
发表时间：
2012-05
期刊：
--
影响因子：
--
作者：
C. Courtney;C. Ong;B. Drinkwater;P. Wilcox;A. Grinenko
通讯作者：
C. Courtney;C. Ong;B. Drinkwater;P. Wilcox;A. Grinenko
影响因子：
12.6
作者：
Dongdong Zhang;Yage Peng;H. Qi;Q. Gao;Chengxiao Zhang
通讯作者：
Dongdong Zhang;Yage Peng;H. Qi;Q. Gao;Chengxiao Zhang
影响因子：
12.6
作者：
Cai, H;Zhu, NN;Fang, YZ
通讯作者：
Fang, YZ