Immunohistochemical localizations of class II antigens and nerve fibers in human carious teeth: HLA-DR immunoreactivity in Schwann cells.
Immunohistochemical localizations of class II antigens and nerve fibers in human carious teeth: HLA-DR immunoreactivity in Schwann cells.
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人类龋齿中 II 类抗原和神经纤维的免疫组织化学定位：雪旺细胞中的 HLA-DR 免疫反应性。
DOI：
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发表时间：
1998
影响因子：
--
通讯作者：
H. Ozawa
中科院分区：
文献类型：
--
作者：
N. Yoshiba;K. Yoshiba;M. Iwaku;H. Ozawa
已知神经纤维和表达II类主要组织相容性复合物（MHC）抗原的树突状细胞聚集在龋损下的牙髓中。重要的功能相互作用可能发生在神经和免疫元件之间。本研究通过HLA-DR单克隆抗体和蛋白基因产物9.5（PGP 9.5）多克隆抗体，共聚焦激光扫描显微镜（CLSM）和电子显微镜，分析了富曼龋牙中II类表达细胞和神经纤维之间的形态关系。在受早期龋齿影响的牙髓中，HLA-DR阳性树突状细胞主要聚集在与PGP 9.5免疫反应神经纤维束相关的无细胞区。在进展期龋损牙髓中，HLA-DR阳性细胞与PGP9.5免疫反应阳性神经纤维紧密相连，尤其在成牙本质细胞层下，HLA-DR阳性细胞与PGP9.5免疫反应阳性神经纤维紧密相连。有趣的是，II类分子不仅在树突状细胞中被识别，而且在牙髓中的无髓鞘神经的雪旺细胞中也被识别。使用免疫电子显微镜，II类分子被定位在非髓鞘形成的许旺细胞的表面上，也在一些囊泡内，而髓鞘形成的许旺细胞缺乏这种免疫反应性。在成牙本质细胞层中也观察到密集的PGP 9.5免疫反应阳性神经纤维，CLSM揭示了神经纤维和树突状细胞的密切联系。雪旺细胞HLA-DR的免疫反应性依赖于龋损的严重程度。表达II类的雪旺氏细胞被认为除了树突状细胞之外还具有抗原呈递细胞的功能。
Nerve fibers and class II major histocompatibility complex (MHC) antigen-expressing dendritic cells have been known to gather in the dental pulp beneath carious lesions. Significant functional interactions presumably occur between the neural and immune elements. The present study analyzed the morphological relationship between class II-expressing cells and nerve fibers in fuman carious teeth, visualized by a HLA-DR monoclonal antibody and a protein gene product 9.5 (PGP 9.5) polyclonal antibody; a confocal laser scanning microscope (CLSM) and an electron microscope were used. In pulps affected by early caries, HLA-DR-positive dendritic cells aggregated mainly in the cell-free zone associated with bundles of PGP 9.5-immuno-reactive nerve fibers. In pulps affected by advanced caries, the accumulated HLA-DR-positive cells and PGP 9.5-immunoreactive nerve fibers showed close association with each other especially beneath the odontoblast layer: the cells even embraced the nerve fibers. Intriguingly, class II molecules were recognized not only in dendritic cells but also in the Schwann cells of non-myelinated nerves in the pulp. Using immuno-electron microscopy, class II molecules were localized on the surface of the non-myelinating Schwann cells and also within some vesicles, whereas myelinating Schwann cells lacked this immunoreactivity. PGP 9.5-immunoreactive nerve fibers were also observed densely in the odontoblast layer, and CLSM revealed an intimate association of the nerve fibers and dendritic cells. The immunoreactivity for HLA-DR in Schwann cells depended upon the severity of the carious lesion. Class II-expressing Schwann cells are suggested to function as antigen-presenting cells in addition to dendritic cells.
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影响因子：
4.4
作者：
Y. Nong;R. Titus;J. Ribeiro;H. Remold
通讯作者：
Y. Nong;R. Titus;J. Ribeiro;H. Remold
影响因子：
56.9
作者：
M. Lotz;J. Vaughan;D. Carson
通讯作者：
M. Lotz;J. Vaughan;D. Carson
影响因子：
3
作者：
P. E. Taylor;M. Byers
通讯作者：
P. E. Taylor;M. Byers
影响因子：
4.2
作者：
BYERS, MR;TAYLOR, PE;KIMBERLY, CL
通讯作者：
KIMBERLY, CL