An R3-MYB repressor, BnCPC forms a feedback regulation with MBW complex to modulate anthocyanin biosynthesis in Brassica napus.
An R3-MYB repressor, BnCPC forms a feedback regulation with MBW complex to modulate anthocyanin biosynthesis in Brassica napus.
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DOI：
10.1186/s13068-022-02227-6
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发表时间：
2022-11-29
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影响因子：
--
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花青素是苯丙素途径的代谢产物，参与植物的多种发育和适应过程，受MYB-bHLH-WD40 （MBW）蛋白复合物的调控。许多R2R3-MYB激活因子已经被很好地表征，但MYB抑制因子在花青素生物合成中也被发现，它们在调节植物苯丙素代谢中也很重要。油料作物甘蓝型油菜花青素生物合成的调控机制尚不清楚。本研究鉴定了甘蓝型油菜花青素抑制因子BnCPC。BnCPC编码了一个典型的R3-MYB蛋白，该蛋白含有一个保守的[D/E]Lx2[R/K]x3Lx6Lx3R基序，用于与bHLH蛋白相互作用。在花青素诱导条件下，过量表达BnCPC可抑制花青素的积累。蛋白-蛋白相互作用和双荧光素酶实验证实，BnCPC可以与BnPAP1竞争，与bHLHs （BnTT8和b忽略3）相互作用，抑制被MBW复合物激活的花青素生物合成基因（如BnDFR）的表达。此外，我们发现BnCPC抑制MBW复合物诱导的BnCPC活性。综上所述，本研究表明BnCPC通过影响MBW复合物的形成来抑制花青素的生物合成，并形成一个反馈回路来调节甘蓝型油菜花青素的积累。在线版本包含补充材料，可在10.1186/s13068-022-02227-6获得。
Anthocyanins are metabolites of phenylpropanoid pathway, and involves in diverse processes of plant development and adaptation, which are regulated by the MYB-bHLH-WD40 (MBW) protein complexes. Many R2R3-MYB activators have been well characterized, but the MYB repressors in anthocyanin biosynthesis were recognized recently, which are also important in modulating phenylpropanoid metabolism in plants. The regulatory mechanism of anthocyanin biosynthesis in oil crop Brassica napus remains to be revealed. In this study, we identified an anthocyanin repressor BnCPC in B. napus. BnCPC encoded a typical R3-MYB protein containing a conserved [D/E]Lx2[R/K]x3Lx6Lx3R motif for interaction with bHLH proteins. Overexpression of BnCPC in B. napus inhibited anthocyanin accumulation, especially under anthocyanin inducible conditions. Protein–protein interaction and dual-luciferase assays confirmed that BnCPC could compete with BnPAP1 to interact with bHLHs (BnTT8 and BnEGL3), and repress the expression of anthocyanin biosynthetic genes (e.g., BnDFR) that activated by MBW complexes. Moreover, we found BnCPC inhibited the MBW complex-induced BnCPC activity. Overall, this research demonstrated that BnCPC repressed anthocyanin biosynthesis by affecting the formation of MBW complex, and formed a feedback loop to regulate anthocyanin accumulation in B. napus. The online version contains supplementary material available at 10.1186/s13068-022-02227-6.
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影响因子：
3.7
作者：
Bac-Molenaar JA;Fradin EF;Rienstra JA;Vreugdenhil D;Keurentjes JJ
通讯作者：
Keurentjes JJ
DOI：
10.1111/j.1365-313x.2006.02964.x
发表时间：
2007-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
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通讯作者：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
7.4
作者：
Cavallini, Erika;Tomas Matus, Jose;Tornielli, Giovanni Battista
通讯作者：
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