Contact-independent killing mediated by a T6SS effector with intrinsic cell-entry properties.
Contact-independent killing mediated by a T6SS effector with intrinsic cell-entry properties.
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由具有内在细胞进入特性的 T6SS 效应器介导的不依赖于接触的杀伤
DOI：
10.1038/s41467-020-20726-8
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发表时间：
2021-01-18
影响因子：
16.6
通讯作者：
Shen X
中科院分区：
文献类型：
--
作者：
Song L;Pan J;Yang Y;Zhang Z;Cui R;Jia S;Wang Z;Yang C;Xu L;Dong TG;Wang Y;Shen X
细菌VI型分泌系统（t6ss）将毒性效应物注入邻近的真核和原核细胞。一般认为，这一过程需要两个细胞之间的物理接触。在这里，我们提供了t6ss分泌的效应物的非接触杀伤的证据。我们发现假结核耶尔森菌利用T6SS （T6SS-3）分泌一种核酸酶效应物，在体外杀死其他细菌，并促进小鼠肠道定植。效应蛋白（Tce1）是一种小蛋白，作为Ca2+和Mg2+依赖的dna酶，其毒性被同源免疫蛋白Tci1抑制。正如预期的那样，T6SS-3通过直接将Tce1注射到邻近细胞中介导典型的接触依赖性杀伤。此外，T6SS-3还通过将Tce1分泌到细胞外环境中，在没有细胞间接触的情况下介导邻近细胞的杀伤。Tce1有效地非接触进入靶细胞需要靶细胞外膜中的OmpF和BtuB蛋白。这种非接触、远距离的T6SS毒素传递的发现，为理解T6SS在竞争中的生理作用提供了新的视角。然而，介导靶细胞非接触摄取Tce1的机制尚不清楚。细菌可以利用VI型分泌系统（t6ss）以接触依赖的方式将毒性效应蛋白注入邻近细胞。在这里，作者提供了T6SS效应物的非接触杀伤的证据，该效应物分泌到细胞外环境中，然后被其他细菌细胞吸收。
Bacterial type VI secretion systems (T6SSs) inject toxic effectors into adjacent eukaryotic and prokaryotic cells. It is generally thought that this process requires physical contact between the two cells. Here, we provide evidence of contact-independent killing by a T6SS-secreted effector. We show that the pathogen Yersinia pseudotuberculosis uses a T6SS (T6SS-3) to secrete a nuclease effector that kills other bacteria in vitro and facilitates gut colonization in mice. The effector (Tce1) is a small protein that acts as a Ca2+- and Mg2+-dependent DNase, and its toxicity is inhibited by a cognate immunity protein, Tci1. As expected, T6SS-3 mediates canonical, contact-dependent killing by directly injecting Tce1 into adjacent cells. In addition, T6SS-3 also mediates killing of neighboring cells in the absence of cell-to-cell contact, by secreting Tce1 into the extracellular milieu. Efficient contact-independent entry of Tce1 into target cells requires proteins OmpF and BtuB in the outer membrane of target cells. The discovery of a contact-independent, long-range T6SS toxin delivery provides a new perspective for understanding the physiological roles of T6SS in competition. However, the mechanisms mediating contact-independent uptake of Tce1 by target cells remain unclear. Bacteria can use type VI secretion systems (T6SSs) to inject toxic effector proteins into adjacent cells, in a contact-dependent manner. Here, the authors provide evidence of contact-independent killing by a T6SS effector that is secreted into the extracellular milieu and then taken up by other bacterial cells.
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影响因子：
4.4
作者：
Jiang, Yu;Chen, Biao;Yang, Sheng
通讯作者：
Yang, Sheng
DOI：
10.1074/mcp.m113.030502
发表时间：
2013-10
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Fritsch MJ;Trunk K;Diniz JA;Guo M;Trost M;Coulthurst SJ
通讯作者：
Coulthurst SJ
影响因子：
30.3
作者：
Ma LS;Hachani A;Lin JS;Filloux A;Lai EM
通讯作者：
Lai EM
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
64.5
作者：
Basler M;Ho BT;Mekalanos JJ
通讯作者：
Mekalanos JJ