Disruption of Intracellular ATP Generation and Tight Junction Protein Expression during the Course of Brain Edema Induced by Subacute Poisoning of 1,2-Dichloroethane.
Disruption of Intracellular ATP Generation and Tight Junction Protein Expression during the Course of Brain Edema Induced by Subacute Poisoning of 1,2-Dichloroethane.
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1,2-二氯乙烷亚急性中毒脑水肿过程中细胞内ATP生成和紧密连接蛋白表达的破坏
DOI：
10.3389/fnins.2018.00012
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发表时间：
2018
影响因子：
4.3
通讯作者：
Jin Y
中科院分区：
文献类型：
--
作者：
Wang G;Yuan Y;Gao L;Tan X;Yang G;Zhao F;Jin Y
探讨1，2-二氯乙烷（1，2-dichloroethane，1，2-DCE）亚急性中毒致脑水肿过程中细胞内ATP生成和紧密连接蛋白表达的变化。将小鼠暴露于1.2 g/m3的1，2-DCE中，每天3.5 h，持续1、2或3 d，即A、B和C组。测定脑组织Na+-K+-ATP酶、Ca 2 +-ATP酶活性、ATP含量、乳酸含量、细胞内游离Ca 2+浓度、ZO-1和occludin表达。结果显示，与对照组相比，B、C组细胞内Ca ~（2+）-ATP酶活性降低，Na ~+/K ~+-ATP酶活性降低，而B、C组细胞内游离Ca ~（2+）浓度升高。与对照组相比，C组ATP含量显著降低，而乳酸含量显著升高。而ZO-1和occludin在B和C组的蛋白和基因表达水平均较对照组显著降低。总之，本研究的结果表明，钙超载和紧密连接相关蛋白，如ZO-1和occludin的表达抑制可能在亚急性中毒1，2-DCE引起的脑水肿形成的早期阶段发挥重要作用。
The aim of this study was to explore changes in intracellular ATP generation and tight junction protein expression during the course of brain edema induced by subacute poisoning of 1,2-dichloroethane (1,2-DCE). Mice were exposed to 1.2 g/m3 1,2-DCE for 3.5 h per day for 1, 2, or 3 days, namely group A, B, and C. Na+-K+-ATPase and Ca2+-ATPase activity, ATP and lactic acid content, intracellular free Ca2+ concentration and ZO-1 and occludin expression in the brain were measured. Results of present study disclosed that Ca2+-ATPase activities in group B and C, and Na+/K+-ATPase activity in group C decreased, whereas intracellular free Ca2+ concentrations in group B and C increased significantly compared with control. Moreover, ATP content decreased, whereas lactic acid content increased significantly in group C compared with control. On the other hand, expressions of ZO-1 and occludin at both the protein and gene levels in group B and C decreased significantly compared with control. In conclusion, findings from this study suggest that calcium overload and depressed expression of tight junction associated proteins, such as ZO-1 and occludin might play an important role in the early phase of brain edema formation induced by subacute poisoning of 1,2-DCE.
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影响因子：
5.6
作者：
Michinaga S;Koyama Y
通讯作者：
Koyama Y
影响因子：
--
作者：
Kojima T;Go M;Takano K;Kurose M;Ohkuni T;Koizumi J;Kamekura R;Ogasawara N;Masaki T;Fuchimoto J;Obata K;Hirakawa S;Nomura K;Keira T;Miyata R;Fujii N;Tsutsumi H;Himi T;Sawada N
通讯作者：
Sawada N
影响因子：
5.1
作者：
Jeremias, Isabela Casagrande;Scaini, Giselli;Streck, Emilio Luiz
通讯作者：
Streck, Emilio Luiz
影响因子：
3.3
作者：
Chen, X.;Threlkeld, S. W.;Cummings, E. E.;Juan, I.;Makeyev, O.;Besio, W. G.;Gaitanis, J.;Banks, W. A.;Sadowska, G. B.;Stonestreet, B. S.
通讯作者：
Stonestreet, B. S.
缓激肽通过下调 ZO-1、occludin 和 claudin-5 的表达水平并重新排列肌动蛋白细胞骨架来增加血液肿瘤屏障通透性
影响因子：
4.2
作者：
Liu, Li-bo;Xue, Yi-xue;Wang, Yi-bao
通讯作者：
Wang, Yi-bao