Accurate membrane tracing in three-dimensional reconstructions from electron cryotomography data.
Accurate membrane tracing in three-dimensional reconstructions from electron cryotomography data.
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DOI：
10.1016/j.ultramic.2015.03.021
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发表时间：
2015-08
期刊：
影响因子：
2.2
通讯作者：
Volkmann, Niels
中科院分区：
文献类型：
--
作者：
Page, Christopher;Hanein, Dorit;Volkmann, Niels
细胞外基质和细胞之间的连接对于力传导和力生物学至关重要。原则上，电子冷冻断层扫描可以对这些连接进行比纳米分辨率更好的分析，但数据收集几何形状的限制阻碍了从这些断层图像中准确提取腹膜位置，而这是分析的重要先决条件。在这里，我们介绍了一种新颖的膜追踪策略，该策略能够以高保真度和非凡的准确性实现腹膜提取。该方法基于检测细胞内部和外部之间的边界，而不是试图明确地追踪细胞膜。模拟研究表明，使用这一原理可以对超过 99% 的膜进行正确建模，并且视觉可识别的膜拉伸与提取的实验数据边界的完美匹配表明其准确性与实际数据相当。
The connection between the extracellular matrix and the cell is of major importance for mechanotransduction and mechanobiology. Electron cryo-tomography, in principle, enables better than nanometer-resolution analysis of these connections, but restrictions of data collection geometry hamper the accurate extraction of the ventral membrane location from these tomograms, an essential prerequisite for the analysis. Here, we introduce a novel membrane tracing strategy that enables ventral membrane extraction at high fidelity and extraordinary accuracy. The approach is based on detecting the boundary between the inside and the outside of the cell rather than trying to explicitly trace the membrane. Simulation studies show that over 99% of the membrane can be correctly modeled using this principle and the excellent match of visually identifiable membrane stretches with the extracted boundary of experimental data indicates that the accuracy is comparable for actual data.
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