Catalase and hydrogen peroxide cytotoxicity in cultured cardiac myocytes.
Catalase and hydrogen peroxide cytotoxicity in cultured cardiac myocytes.
复制标题
培养的心肌细胞中过氧化氢酶和过氧化氢的细胞毒性。
DOI：
10.1016/0022-2828(95)90041-1
复制
发表时间：
1995
影响因子：
5
通讯作者：
Leff,JA
中科院分区：
文献类型：
--
作者：
Horwitz,LD;Leff,JA
We examined the role of intracellular catalase activity in modulating hydrogen peroxide (H2O2)-induced cytotoxicity in cultured chick embryo cardiac myocytes. Injury was quantitated by release of lactate dehydrogenase (LDH). Application of 1.5 mm H2O2to myocytes caused LDH release beginning at 2 h. Inactivation or inhibition of catalase with aminotriazole or sodium azide increased LDH release but did not cause earlier release. Free catalase which entered or became associated with myocytes, but not catalase bound to agarose beads, which did not enter or become associated with myocytes, was protective. Separate experiments demonstrated that myocyte catalase activity decreased by 27% between 1 and 4 h of H2O2exposure. Treatment with aprotinin, a protease inhibitor, prevented the H2O2-induced fall in catalase activity at 4 h but treatment with deferoxamine, an iron chelator, had no effect on catalase activity. Thus, with exposure of cardiac myocytes to H2O2, the magnitude of the cytotoxicity is modulated by endogenous or cell associated exogenous catalase. It is proposed that in addition to excessive accumulation of H2O2, a reduction in intracellular catalase activity may be required before substantial cell injury occurs during H2O2exposure. Activation of proteases may cause the reduction in catalase activity in this setting.
登录
查看更多内容
影响因子：
--
作者：
BYLER, RM;SHERMAN, NA;HORWITZ, LD
通讯作者：
HORWITZ, LD
影响因子：
5
作者：
GUARNIERI, C;FLAMIGNI, F;CALDARERA, CM
通讯作者：
CALDARERA, CM
自由基作为组织损伤的介质
DOI：
10.1007/978-1-4612-0419-0_2
发表时间：
1980
期刊：
Acta Physiologica Scandinavica
影响因子：
--
作者：
J. McCord;K. Wong;Stokes Sh;W. F. Petrone;D. English
通讯作者：
D. English
影响因子：
37.8
作者：
Quaife,RA;Kohmoto,O;Barry,WH
通讯作者：
Barry,WH
人血清过氧化氢酶可减少过氧化氢造成的内皮细胞损伤。
DOI：
10.1152/jappl.1991.71.5.1903
发表时间：
1991
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Leff,JA;Oppegard,MA;Terada,LS;McCarty,EC;Repine,JE
通讯作者：
Repine,JE